货号：AB960

品牌：Merck Millipore

规格：

目录价：￥3615.00

市场价格：￥3072.75

会员价格：￥2892.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Prolactin Antibody | AB960 View All» Replaces: CBL78 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: The antiserum provided has been evaluated for its ability to give a specific and positive immunostaining of prolactin-containing cells using both the immunoperoxidase and immunofluorescence procedures. View All» Immunogen: ANTIGEN: The antigen used in the production of the antiserum has been purified from sheep pituitary glands. View All» Species Reactivity:

• Bovine

• Canine

• Human

• Rat

• Sheep

• Feline
  View All» Application Notes: TISSUE EVALUATION: Formalin-fixed and paraffin-embedded sections (6 microns thick) of anterior pituitary glands were prepared from a number of species including human, dog, rat, cat, ox and sheep. Cryostat sections (10 microns thick) of the above tissues can also be used in the staining procedures.IMMUNOPEROXIDASESTAINING:1. The sections to be stained are hydrated to phosphate buffered saline (PBS, pH 7.4).2. The antiserum diluted 1:1,000 - 1:2,000 (in PBS) is allowed to react with the tissue at 37°C for 30 minutes.3. Using PBS, the tissue is washed for 15 minutes at room temperature and treated sequentially with sheep anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 37°C. After each of these two steps excess reagent is washed from the section as described above.4. The final step in the staining involves treating the section with a mixture of 3,3'-diaminobenzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted.IMMUNOFLUORESCENCESTAINING:1. Same as Step 1 above.2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 minutes at 37°C.3. After washing off the excess reagents with PBS, the section is treated with fluorescein labeled goat anti-rabbit gamma globulin (diluted 1:10 in PBS) for 30 minutes at 37° C. The tissue is washed with PBS and after mounting under glycerine-saline is viewed with a fluorescence microscope.COMMENTS:The antiserum dilutions described have been established as giving a positive staining on the lactotrophs when using the above protocol. It is recommended, however, that the customer establishes his own optimal dilutions to give positive staining. In general, staining can occur on further dilution of the primary antiserum if this is accompanied by an increase in its incubation time e.g. 24 hr or 4° C. View All» Presentation: Liquid antiserum. Contains 0.1% sodium azide. View All» Storage Conditions: Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. View All» UniProt Number: P01236 View All» Entrez Gene Number: NM_000948.3 View All» Gene Symbol:
  • PRL

  • prolactin
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunofluorescence

    • Immunohistochemistry (Paraffin)
      View All» UniProt Summary: FUNCTION: SwissProt: P01236 # Prolactin acts primarily on the mammary gland by promoting lactation.SIZE: 227 amino acids; 25876 Da SUBCELLULAR LOCATION: Secreted.SIMILARITY: SwissProt: P01236 ## Belongs to the somatotropin/prolactin family. View All» Brand Family: Chemicon View All» Product Name: Anti-Prolactin Antibody | AB960 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 500 µL View All» Format: Serum View All» Host: Rabbit View All»
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