货号：AB9644

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Dab1 phospho Tyrosine 198 Antibody | AB9644 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Dab1 phosphoTyrosine 198. The antibody recognizes a protein of ~80 kDa corresponding to Dab1 pTyrosine 198 in lysates from HEK293 transfected cells. View All» Immunogen: Synthetic peptide of amino acids surrounding the phosphoTyrosine 198 site of human Dab1. View All» Modifications: Phosphorylation View All» Background Information: Dab1 is a 80 kDa protein that is encoded by the Disabled-1 gene locus which is mutated in scrambler and yotari mutant mice. Phenotypically mutatation of this gene produces motor defects and ataxia, disruption of neuronal migration, and severe cerebellar hypoplasia. Dab1 is an intracellular adapter protein that functions in downstream signaling events initiated by reelin. Dab1 phosphorylation at tyrosine 198 is a major site for reelin-induced Src family kinase-mediated phosphorylation in embryonic neurons. View All» Species Reactivity: Mouse View All» Application Notes: Western blot: 1:1,000. For use in detecting endogenous Dab1 protein it is recommended that the Dab1 protein first be immunoprecipitated. Suggested blocking buffer is 5% BSA-TBST. Suggested blocking time is overnight at 2-8?C. Suggested antibody dilution buffer is 3% BSA-TBST. Suggested antibody incubation time is 2 hours at room temperature. Optimal working dilutions must be determined by the end user. View All» Control: POSITIVE CONTROL: HEK293 cells transfected with wild-type mouse Dab1 protein. View All» Presentation: Protein A purified immunoglobulin. Liquid in Dulbecco's PBS (without Mg2+ and Ca2+), pH 7.3, 50% glycerol with 1.0 mg/mL BSA and 0.05% sodium azide. View All» Storage Conditions: Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer. View All» UniProt Number: O75553 View All» Entrez Gene Number: NM_021080.3 View All» Gene Symbol: DAB1 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice, the disabled-1 gene plays a central role in brain development, directing the migration of cortical neurons past previously formed neurons to reach their proper layer. This gene is similar to disabled-1, and the protein encoded by this gene is thought to be a signal transducer that interacts with protein kinase pathways to regulate neuronal positioning in the developing brain. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. View All» UniProt Summary: FUNCTION: SwissProt: O75553 # Adapter molecule functioning in neural development. May regulate SIAH1 activity (By similarity).SIZE: 588 amino acids; 63775 Da SUBUNIT: Associates with the SH2 domains of SRC, FYN and ABL. Interacts with DAB2IP and SIAH1 (By similarity). Interacts with LRP1.DOMAIN: SwissProt: O75553 The PID domain specifically binds to the Asn-Pro-Xaa- Tyr(P) motif found in many tyrosine-phosphorylated proteins.PTM: Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons (By similarity). Also phosphorylated on Ser- 524 independently of reelin signaling (By similarity).SIMILARITY: Contains 1 PID domain. View All» Brand Family: Chemicon View All» Product Name: Anti-Dab1 phospho Tyrosine 198 Antibody | AB9644 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Purified View All» Host: Rabbit View All»