货号：AB9728

品牌：Merck Millipore

规格：100Ug

目录价：￥6127.00

市场价格：￥5207.95

会员价格：￥4901.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-S100A12 Antibody | AB9728 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: By western blot this polyclonal antibody recognizes a band at ~10-11 kDa under reducing conditions. A dimer can be sometimes be seen at ~ 21 kDa using recombinant human S100A12. Antibody was affinity purifed over immobilized S100A12 prior to vialing. View All» Immunogen: Full length recombinant His-tagged human S100A12 that was expressed in E. coli and purified by Ni-NTA affinity chromatography prior to immunization. View All» Background Information: S100 calcium binding protein A12, also known as S100A12, EN-RAGE, and calgranulin C, is a proinflammatory protein with significant potential as a biomarker. It is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. The member S100A12 is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. It has been reported to be secreted by activated granulocytes and binds to the receptor for advanced gycation end products, which induces NfkappaB-dependent activation of endothelium (Foell et al., 2003). S100A12 is strongly expressed in inflamed synovial tissue typical in rheumatoid and psoriatic arthritis and may also prove an excellent biomarker for Inflammatory bowel disease (Kaiser et al., 2007). S100A12 protein may also be involved in chronic inflammation in atherosclerotic lesions including those of Type II diabetics (Kosaki et al. 2004) and the acceleration of atherosclerosis in hemodialysis patients (Mori et al., 2009).Foell, D. et al. (2003). Expression of the pro-inflammatory protein S100A12 (EN-RAGE) in rheumatoid and psoriatic arthritis. Rheumatol. 42:1383-1389. Kaiser, T. et al. (2007). Faecal S100A12 as a non-invasive marker distinguishing inflammatory bowel disease from irritable bowel syndrome. Gut. 56:1706-1713. Kosaki, A. et al. (2004). Increased plasma S100A12 (EN-RAGE) levels in patients with type 2 diabetes. J. Clin. Endocrinol. Metabol. 89:5423-5428. Mori, Y. et al. (2009). Increased Plasma S100A12 (EN-RAGE) levels in hemodialysis patients with atherosclerosis. Am. J. Nephrol. 29:18-24. View All» Species Reactivity: Human View All» Application Notes: Western blot: 1:1,000-1:10,000 on recombinant human S100A12.Immunohistochemistry: fesh frozen, acetone fixed sections 1:100-1:500 using ABC enzymatic detection methods (Srikrishna, G. et al, 2005). Other fixation conditions not yet tested. ELISA: 1:10,000-1:100,000 against the recombinant human S100A12.It is expected that the antibody will also work for immunohistochemistry.Optimal working dilutions must be determined by the end user. View All» Control: HL-60 cells, and human PMN/Granulocytes {Vogl, T et al (1999) “S100A12 Is Expressed Exclusively by Granulocytes and Acts Independently from MRP8 and MRP14” J. Biol. Chem. 274(3):25291-25296}. View All» Presentation: Liquid in PBS with 0.1% sodium azide. View All» Storage Conditions: Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. View All» UniProt Number: P80511 View All» Alternate Names:

• EN-RAGE

• Extacellular newly identified RAGE-binding protein

• CAAF1

• Calcium-binding protein in amniotic fluid-1

• Calgranulin C

• Calgranulin-related protein

• CGRP

• Cornea-associated antigen

• CO-Ag
  View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
  • ELISA

  • Immunohistochemistry

  • Western Blotting
    View All» Brand Family: Chemicon View All» Product Name: Anti-S100A12 Antibody | AB9728 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Affinity Purified View All» Host: Rabbit View All»
    © 金山科研平台是专业的授权总代理区域代理经销平台。
    © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
    © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
    © 本文地址：https://www.16ao.com/thread-158643.htm
    © Merck Millipore代理AB9728 Anti-S100A12 Antibody;store at -20℃，产品报价联系微信jinshanbio