货号：AB982

品牌：Merck Millipore

规格：

目录价：￥3908.00

市场价格：￥3321.80

会员价格：￥3126.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Vasoactive Intestinal Peptide Antibody | AB982 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: The antiserum supplied has been evaluated for its ability to stain ganglion cells in both the superficial and deep plexus of the wall of the human small bowel. View All» Immunogen: Synthetic (18-28) porcine vasoactive intestinal peptide bound to keyhole limpet hemocyanin (KLH) with carbodiimide. View All» Species Reactivity:

• Canine

• Guinea Pig

• Human

• Mouse

• Rat

• Feline
  View All» Application Notes: TISSUE EVALUATION: Formalin or alcohol fixed and paraffin-embedded sections (7-8 microns thick) of small bowel tissue prepared from a number of species including human, rat, cat, dog and guinea pig. Cryostat sections of the above tissue can also be used in the staining procedures. IMMUNOPEROXIDASE STAINING: 1. The sections to be stained are hydrated to phosphated buffered saline (PBS, pH 7.4). 2. The antiserum diluted 1:500-1:1,000 (in PBS) is allowed to react with the tissue at 37°C for 30 minutes. Optimal dilution must be determined by the end user. 3. Using PBS, the tissue is washed for 15 minutes at room temperature and treated sequentially with goat anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 30 minutes at 37°C. After each of these steps, the tissue is washed with PBS as before. 4. The final stage of the reaction is treatment of the section with a mixture of 3,3'-diamino-benzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated through alcohol, cleared in xylene and mounted. IMMUNOFLUORESCENT STAINING: 1. Same as Step 1 above. 2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 minutes at 37°C. Optimal dilution must be determined by the end user. 3. After washing off the excess antiserum with PBS, the section is treated with fluorescein-labeled goat anti-rabbit gamma globulin serum (diluted 1:10 in PBS) for 30 minutes at 37°C. The tissue is washed with PBS and after mounting under saline-glycerol, is viewed with a fluorescence microscope. COMMENTS: The antiserum described here has been established to give a positive staining of gastrin cells using the above protocol. It is recommended, however, that the customer establishes his own optimal dilutions to give positive staining. In general, staining can occur on further dilution of the primary antiserum if this is accompanied by an increase in its incubation time, e.g. 24 hours at either 37*C or 4*C. View All» Control: Small bowel tissue with plexus attached View All» Purification Method: Unpurified View All» Presentation: Liquid antiSerum containing 0.1% sodium azide. View All» Storage Conditions: Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. View All» UniProt Number: P01282 View All» Entrez Gene Number:
  • NM_003381.2

  • NM_194435.1
    View All» Gene Symbol:
    • VIP

    • MGC13587

    • PHM27
      View All» Alternate Names: VIP View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Immunohistochemistry (Paraffin) View All» Entrez Gene Summary: The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. View All» UniProt Summary: FUNCTION: SwissProt: P01282 # PHM and PHV also cause vasodilation.SIZE: 170 amino acids; 19169 Da SUBCELLULAR LOCATION: Secreted.SIMILARITY: SwissProt: P01282 ## Belongs to the glucagon family. View All» Brand Family: Chemicon View All» Product Name: Anti-Vasoactive Intestinal Peptide Antibody | AB982 View All» Concentration: Variable View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μL View All» Format: Serum View All» Host: Rabbit View All»
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