货号：AB9864

品牌：Merck Millipore

规格：50UL

目录价：￥5974.00

市场价格：￥5077.90

会员价格：￥4779.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-NMDAR1 Antibody | AB9864 View All» Replaces: AB1516 and 07-362 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: The original sequence used is selective for splice variants NR1-1a, NR1-1b, NR1-2a, NR1-2b using the nomenclature defined by Hollmann, M. et al. (1993). These appear to be the major splice variants expressed in rat brain (Sugihara H, et al., 1992). No cross-reaction with other glutamate receptor subunits. Western Blot analysis shows that this rabbit monoclonal antibody labels a single band at approximately 120 kD corresponding to NMDAR1 in rat brain lysate. View All» Molecular Weight: 120 kDa View All» Epitope: a.a. 909-938 View All» Immunogen: Synthetic peptide corresponding to the C-terminus of rat NMDA receptor subunit (Catalog number AG344). {amino acids 909-938 rat NMDAR1{LQNQKDTVLPRRAIERE EGQLQLCSRHRES} View All» Clone: 1.17.2.6 View All» Isotype: IgG View All» Background Information: N-methyl-D-aspartate Receptors (NMDAR) are one of three pharmacologically distinct subtypes of ionotropic receptors that mediate a majority of excitatory neurotransmission in the brain via the endogenous amino acid, L-glutamate. MDARs form heteromeric complexes usually composed of two components, the NR1 subunit and NR2 subunits. Both proteins are weakly expressed throughout the brain but show localization in the dendritic region of the mushroom bodies. The NMDAR channel is highly permeable to Ca2+ and Na+ and its opening requires the simultaneous binding of glutamate and postsynaptic membrane depolarization. The activated NMDAR channel allows calcium influx into the post-synaptic cell where calcium triggers a cascade of biochemical events that create synaptic changes. NMDAR have been studied extensively in Drosophila for its potential relationship to behavioral plasticity. A newly identified NMDAR complex of more than 80 different proteins has been identified. Genetic and pharmacological disruptions of components of the NMDAR complex produce learning impairments in rodents, further linking behavioral plasticity to synaptic plasticity. View All» Species Reactivity: Rat View All» Species Reactivity Note: Reactivity in mouse is weak. On reduced Western blots a band matching that in rat is observed but tests in immunohistochemistry have been unsuccessful. It is expected that the antibody will also react with monkey, gerbil, rabbit, feline and fish based on the fact that another antibody made to the same peptide sequence reacts with those species. View All» Application Notes: Immunohistochemistry: AB9864 can be used for using 4% paraformaldehyde or paraformaldehyde/glutaraldehyde fixed rat tissue. Suggested working dilution for light microscopy is 1:1,000-1:2,000.Western blot: 1:1,000-1:2,000 using ECL on rat brain lysate.Optimal working dilutions must be determined by end user. View All» Control: Brain tissue View All» Quality Assurance: Routinely evaluated by Western Blot on rat brain lysates.Western Blot Analysis: 1:1000 dilution of this lot detected NMDAR1 on 10 μg of rat brain lysates View All» Purification Method: Unpurified View All» Presentation: Rabbit monoclonal supernatant liquid containing 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. View All» UniProt Number: Q05586 View All» Entrez Gene Number: NM_000832.5 View All» Gene Symbol:

• GRIN1

• NR1

• Glutamate

• NMDAR

• NMDA1

• NMDAR1
  View All» Alternate Names:
  • N-methyl-D-aspartate receptor channel, subunit zeta-1

  • N-methyl-D-aspartate receptor subunit NR1

  • NMDA receptor 1

  • glutamate [NMDA] receptor subunit zeta 1

  • glutamate receptor, ionotropic, N-methyl D-aspartate 1
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunohistochemistry

    • Western Blotting
      View All» Entrez Gene Summary: The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described. View All» UniProt Summary: FUNCTION: SwissProt: Q05586 # NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors (By similarity).SIZE: 938 amino acids; 105373 Da SUBUNIT: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B); disulfide-linked. Found in a complex with GRIN2A or GRIN2B, GRIN3A or GRIN3B and PPP2CB. Interacts with DLG4 and MPDZ (By similarity).SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity). Note=Enriched in post-synaptic plasma membrane and post-synaptic densities (By similarity).PTM: NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity (By similarity).SIMILARITY: SwissProt: Q05586 ## Belongs to the glutamate-gated ion channel (TC 1.A.10) family View All» Brand Family: Chemicon View All» Product Name: Anti-NMDAR1 Antibody | AB9864 View All» Concentration: Variable View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 50 µL View All» Format: Culture Supernatant View All» Host: Rabbit View All»
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