货号：ABC27

品牌：Merck Millipore

规格：100UL

目录价：￥4146.00

市场价格：￥3524.10

会员价格：￥3316.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-ICAD (DFF) (cleaved form) Antibody | ABC27 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes the endogenous, cleaved form of ICAD (DFF). View All» Molecular Weight: ~11 kDA observed. In Western Blot, ICAD is shown to be cleaved into ~10-15 kDa and ~24-30 kDa fragments (Todor, A., et al. (2002). Am J Physiol Heart Circ Physiol. 283:H990-H995; Liu, X., et al. (1997). Cell. 89:175-184). View All» Immunogen: KLH-conjugated linear peptide corresponding to the endogenous, cleaved form of human ICAD (DFF). View All» Background Information: Apoptosis is related to many diseases and is induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These cell death signals cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis (Liu et al. 1997). The mouse homologue of this human DFF was identified as a DNase inhibitor designated ICAD (Enari et al. 1998; Sakahira et al. 1998). Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released, activated and eventually causes the degradation of DNA in the nuclei (Enari et al. 1998; Sakahira et al. 1998). Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is identified as a hallmark of apoptotic cell death (Wylile et al. 1998). View All» Species Reactivity: Human View All» Species Reactivity Note: Demonstrated to react with Human. View All» Application Notes: Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected cleaved ICAD (DFF) in staurosporin-treated HeLa cells. View All» Control: Untreated and staurosporin-treated HeLa cell lysate View All» Quality Assurance: Evaluated by Western Blot in HeLa cell lysate untreated and staurosporin-treated.Western Blot Analysis: A 1:500 dilution of this antibody detected cleaved ICAD (DFF) in untreated and staurosporin-treated HeLa cell lysate. View All» Purification Method: Affinity Purified View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: O00273 View All» Entrez Gene Number: NP_004392 View All» Gene Symbol:

• DFF1

• DFFA

• DFF45

• H13

• DFF-45
  View All» Alternate Names:
  • ICAD

  • DFF-45

  • DNA fragmentation factor subunit alpha

  • DNA fragmentation factor 45 kDa subunit

  • Inhibitor of CAD, DNA degradation of chromosomal DNA, DNase
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunocytochemistry
      View All» Entrez Gene Summary: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. View All» UniProt Summary: FUNCTION: Inhibitor of the caspase-activated DNase (DFF40).SUBUNIT STRUCTURE: Heterodimer of DFFA and DFFB.SUBCELLULAR LOCALIZATION: Cytoplasm. View All» Product Name: Anti-ICAD (DFF) (cleaved form) View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Affinity Purified View All» Host: Rabbit View All»
      © 金山科研平台是专业的授权总代理区域代理经销平台。
      © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
      © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
      © 本文地址：https://www.16ao.com/thread-158789.htm
      © Merck Millipore代理ABC27 Anti-ICAD (DFF) (cleaved form);store at +2℃ to +8℃，产品报价联系微信jinshanbio