货号：ABC35

品牌：Merck Millipore

规格：100Ug

目录价：￥4563.00

市场价格：￥3878.55

会员价格：￥3650.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-HRS (HGS) Antibody | ABC35 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes HRS at the interaction with SNX1 region. View All» Molecular Weight: ~110 kDa observed. An uncharacterized band appears at ~37 kDa in some lysates. The calculated molecular weight is 86 kDa, however HRS has been shown as a ~110 kDa band in western blots. (Urbè, S., et al (2000). Molecular and Cellular Biology. 7685–7692.) View All» Epitope: Interaction with SNX1 region View All» Immunogen: KLH-conjugated linear peptide corresponding to the interaction with SNX1 region of human HRS. View All» Background Information: Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS), otherwise known as HGS or Protein pp110, is a ubiquitously expressed zinc finger protein. HRS is heavily phosphorylated by receptor tyrosine kinases in response to signals from growth factors. It has been detected in vesicular structures and early endosomes and plays a crucial role in endocytosis and vesicle trafficking, which may underlie the downregulation of growth factor receptors such as the epidermal growth factor receptor (EGFR). In order to perform this function, HRS is ubiquitinated at its ubiquitin interacting motif (UIM). This allows interaction of HRS with similarly ubiquitinated vesicle-related proteins such as STAM1 and Eps15, as well as ubiquitinated receptors tagged for lysosomal degradation. Complexes of HRS and STAM1 are located in vesicular membranes and regulate the sorting of tagged receptors to the lysosomes. View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Demonstrated to react in Human and Mouse. Predicted to react with Rat and Bovine based on 100% sequence homology. Other homologies: Xenopus and Zebrafish (94% sequence homology). View All» Control: EGF treated HepG2 cell lysate View All» Quality Assurance: Evaluated by Western Blot in EGF treated HepG2 cell lysate.Western Blot Analysis: 0.1 µg/mL of this antibody detected HRS on 10 µg of EGF treated HepG2 cell lysate. View All» Purification Method: Affinity purified View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: O14964 View All» Entrez Gene Number: NP_004703 View All» Gene Symbol:
  • HGS

  • HRS
    View All» Alternate Names:
    • Hepatocyte growth factor-regulated tyrosine kinase substrate

    • Hrs

    • Protein pp110

    • HRS
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: The protein encoded by this gene regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation. [provided by RefSeq, Dec 2010]. View All» UniProt Summary: FUNCTION: Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.SUBUNIT STRUCTURE: Interacts with TRAK1. Interacts with TRAK2 (By similarity). Interacts with TRAK1. Component of the ESCRT-0 complex composed of STAM or STAM2 and HGS. Part of a complex at least composed of HSG, STAM2 (or probably STAM) and EPS15. Interacts with STAM. Interacts with STAM2. Interacts with EPS15; the interaction is direct, calcium-dependent and inhibited by SNAP25. Interacts with NF2; the interaction is direct. Interacts with ubiquitin; the interaction is direct. Interacts with VPS37C. Interacts with SMAD1, SMAD2 and SMAD3. Interacts with TSG101; the interaction mediates the association with the ESCRT-I complex. Interacts with SNAP25; the interaction is direct and decreases with addition of increasing concentrations of free calcium. Interacts with SNX1; the interaction is direct. Component of a 550 kDa membrane complex at least composed of HGS and SNX1 but excluding EGFR. Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3. SUBCELLULAR LOCATION: Cytoplasm. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Endosome › multivesicular body membrane; Peripheral membrane protein (By similarity). TISSUE SPECIFICITY: Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes. DOMAIN: Has a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix. The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns3P-enriched membranes is substantially increased in acidic conditions. PTM: Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected (By similarity). Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF. Ubiquitinated by ITCH. SEQUENCE SIMILARITIES: Contains 1 FYVE-type zinc finger.Contains 1 UIM (ubiquitin-interacting motif) repeat.Contains 1 VHS domain. View All» Product Name: Anti-HRS (HGS) View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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