货号：ABD78

品牌：Merck Millipore

规格：

目录价：￥4439.00

市场价格：￥3773.15

会员价格：￥3551.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Dnmt3l Antibody | ABD78 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes domain of ADD of Dnmt3l. View All» Molecular Weight: ~52 kDa observed. Uniprot describes two isoforms at ~44 kDa each produced by alternative splicing. Dnmt3l has been observed at ~50 kDa in some cell extracts. (Nimura, K., et al. (2006). Genes Cells. 11(10):1225-1237). View All» Epitope: Domain of ADD. View All» Immunogen: KLH-conjugated linear peptide corresponding to the domain of ADD of human Dnmt3l. View All» Background Information: Methylation of DNA plays an important role in the regulation of gene expression in eukaryotic cells. This process relies on the activity of DNA methyltransferases, including DNMT3A and DNMT3B. The DNMT3L (DNA (cytosine-5)-methyltransferase 3-like) protein shares sequence homology with the DNMT3 family, but lacks a catalytic domain. DNMT3L regulates DNA methylation by binding to the catalytic domains of DNMT3A and DNMT3B thus enabling more rapid binding of these proteins to DNA and cofactors such as AdoMet. Previous studies have demonstrated that DNMT3L stimulates de novo methylation of DNA by activating murine Dnmt3L; and under those conditions, methylation was observed at specific maternal imprinting centers. View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Demonstrated to react with Human and Mouse. Other homologies: Rat (83% sequence homology). View All» Application Notes: Western Blot Analysis: A representative lot of this antibody detected Dnmt3l in human testis tissue lysate, and in H9 and mESC cell lysates. View All» Control: Mouse testis tissue lysate View All» Quality Assurance: Evaluated by Western Blot in mouse testis tissue lysate.Western Blot Analysis: 1 µg/mL of this antibody detected Dnmt3l in 10 µg of mouse testis tissue lysate. View All» Purification Method: Affinity purified View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q9UJW3 View All» Entrez Gene Number: NP_037501 View All» Gene Symbol: DNMT3L View All» Alternate Names: DNA (cytosine-5)-methyltransferase 3-like View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternative splicing results in two transcript variants. An additional splice variant has been described but its biological validity has not been determined. View All» UniProt Summary: FUNCTION: Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3.SUBUNIT STRUCTURE: Homodimer. Heterotetramer composed of 1 DNMT3A homodimer and 2 DNMT3L subunits (DNMT3L-DNMT3A-DNMT3A-DNMT3L). Interacts with histone H3 (via N-terminus).SUBCELLULAR LOCATION: Nucleus.TISSUE SPECIFICITY: Expressed at low levels in several tissues including testis, ovary, and thymus.MISCELLANEOUS: Interaction with histone H3 is strongly inhibited by methylation at lysine 4 (H3K4me).SEQUENCE SIMILARITIES: Belongs to the C5-methyltransferase family. Contains 1 ADD domain. Contains 1 GATA-type zinc finger. Contains 1 PHD-type zinc finger. View All» Product Name: Anti-Dnmt3l View All» Concentration: 1.0 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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