货号：ABE257

品牌：Merck Millipore

规格：100UL

目录价：￥4438.00

市场价格：￥3772.30

会员价格：￥3550.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-RAD51 Antibody | ABE257 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Molecular Weight: ~37 kDa observed. Two isoforms (isoform 1 at 37 kDa and isoform 2 at 26 kDa) produced as a result of alternative splicing are known to exist: . View All» Immunogen: Histidine-tagged recombinant protein corresponding to human RAD51. View All» Background Information: RAD51 is essential for homologous recombination of DNA during repair of double-strand breaks. The N-terminus of RAD51 functions to bind DNA and promote homologous pairing and strand exchange within a regular nucleoprotein filament. In yeast, the Rad51 family of related proteins also includes Rad55, Rad57, and Dmc1. In mammalian cells, seven genes in this family have been identified (HsRAD51, XRCC2, XRCC3, RAD51B/hREC2 and HsDMC1, RAD51C, and RAD51D). RAD51 is predominantly expressed in the testis and thymus but can also be found in the small intestine, placenta, colon, pancreas and ovaries. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Demonstrated to react with Human, Mouse, and Rat. Other homologies: Bovine, Equine, and Canine (99% sequence homology). View All» Application Notes: Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected RAD51 in A431 and HeLa cells.Immunoprecipitation Analysis: 40 µLof Anti-RAD51 immunoprecipitated RAD51 from HeLa RIPA lysate. View All» Control: HeLa cell lysate View All» Quality Assurance: Evaluated by Western Blot in HeLa cell lysate.Western Blot Analysis: 1:2,500 dilution of this antibody detected RAD51 on 10 µg of HeLa cell lysate. View All» Purification Method: Affinity purified View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q06609 View All» Entrez Gene Number: NP_002866 View All» Gene Symbol: RAD51 Synonyms: RAD51A, RECA View All» Alternate Names: DNA repair protein RAD51 homolog 1Short name=HsRAD51Short name=hRAD51Alternative name(s):RAD51 homolog A View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
  • Western Blotting

  • Immunocytochemistry

  • Immunoprecipitation
    View All» Entrez Gene Summary: The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.SUBUNIT STRUCTURE: Interacts with BRCA1, BRCA2 and either directly or indirectly with p53. Interacts with XRCC3, RAD54L and RAD54B. Part of a complex with RAD51C and RAD51B. Interacts with RAD51AP1 and RAD51AP2. Interacts with CHEK1/CHK1, and this may require prior phosphorylation of CHEK1. Interacts with the MND1-PSMC3IP heterodimer (By similarity). Interacts with OBFC2B. Interacts with RPA1. Interacts with PALB2. Interacts with SWI5/C9orf119, and at lower level with SFR1/MEIR5. SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Cytoplasm › perinuclear region. Mitochondrion matrix. Note: Colocalizes with RAD51AP1 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. TISSUE SPECIFICITY: Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.INDUCTION: Stress-induced increase in the mitochondrial levels is seen. PTM: Phosphorylated. Phosphorylation of Thr-309 by CHEK1/CHK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. INVOLVEMENT IN DISEASE: Defects in RAD51 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case. Ref.28SEQUENCE SIMILARITIES: Belongs to the RecA family. RAD51 subfamily.Contains 1 HhH domain. View All» Product Name: Anti-RAD51 View All» Concentration: 0.25 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Affinity Purified View All» Host: Rabbit View All»
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