货号：ABE33

品牌：Merck Millipore

规格：

目录价：￥4621.00

市场价格：￥3927.85

会员价格：￥3696.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-Chk2 (Thr68) Antibody | ABE33 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: Recognizes Chk2 phosphorylated at Thr68. View All» Molecular Weight: The observed molecular weight is 61 kDa. UniProtKB/Swiss-Prot O96017 (CHK2_HUMAN) describes 12 isoforms produced by alternative splicing. View All» Epitope: Thr68 View All» Immunogen: KLH-conjugated linear peptide corresponding to human Chk2 phosphorylated at Thr68. View All» Background Information: Chk2 is a protein kinase activated and phosphorylated in response to DNA damage, especially DNA double-strand breaks. As a cell cycle checkpoint regulator it acts to prevent DNA replication and inhibit mitotic entry prior to the conclusion of DNA replication. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene mutated in breast and ovarian cancer.There are 12 known isoforms of Chk2. The majority of which are expressed solely in the cell nucleus. The exception is isoform 10 which is found throughout the cell. High expression has been observed in spleen, peripheral blood leukocytes, colon and testis. Lower expression is observed in other tissues. Mutations in CHEK2 gene coding for Chk2 are associated with Li-Fraumeni syndrome 2, and have been observed in some patients that contracted prostate cancer and osteosarcoma. View All» Species Reactivity:

• Human

• Pig

• Chimpanzee

• Rhesus Macaque
  View All» Species Reactivity Note: Demonstrated to react with human. Predicted to react with pig, chimpanzee, and rhesus monkey based on 100% sequence homology. View All» Application Notes: Western Blot Analysis: 1 µg/ml from a representative lot detected Chk2 on 10 µg of HEK293 cell lysate. View All» Control: HeLa cell lysate View All» Quality Assurance: Evaluated by Western Blot in HeLa cell lysate.Western Blot Analysis: 0.5 µg/ml of this antibody detected Chk2 on 10 µg of HeLa cell lysate. View All» Purification Method: Affinity Purfied View All» Presentation: Purified rabbit polyclonal containing PBS with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: O96017 View All» Entrez Gene Number: NP_009125 View All» Gene Symbol:
  • CHEK2

  • CDS1

  • CHK2

  • Cds1

  • HuCds1

  • LFS2

  • PP1425

  • RAD53
    View All» Alternate Names:
    • CHK2 (checkpoint, S.pombe) homolog

    • CHK2 checkpoint homolog (S. pombe)

    • checkpoint-like protein CHK2

    • protein kinase CHK2

    • serine/threonine-protein kinase CHK2
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. View All» UniProt Summary: FUNCTION:Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.COFACTOR:Magnesium.ENZYME REGULATION: Rapidly phosphorylated on Thr-68 by MLTK in response to DNA damage and to replication block. Kinase activity is also up-regulated by autophosphorylation. SUBCELLULAR LOCATION: Isoform 2: Nucleus. Note: Isoform 10 is present throughout the cell.Isoform 4: Nucleus. Isoform 7: Nucleus. Isoform 9: Nucleus. Isoform 12: Nucleus. TISSUE SPECIFICITY: High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.PTM: Phosphorylated by PLK4. INVOLVEMENT IN DISEASE: Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.Defects in CHEK2 are found in some patients with prostate cancer (CaP) [MIM:176807].Defects in CHEK2 are found in some patients with osteosarcoma (OSRC) [MIM:259500].SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.Contains 1 FHA domain.Contains 1 protein kinase domain. View All» Product Name: Anti-phospho-Chk2 (Thr68) View All» Concentration: 0.5 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
      © 金山科研平台是专业的授权总代理区域代理经销平台。
      © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
      © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
      © 本文地址：https://www.16ao.com/thread-159055.htm
      © Merck Millipore代理ABE33 Anti-phospho-Chk2 (Thr68)，产品报价联系微信jinshanbio