货号：ABE375

品牌：Merck Millipore

规格：100Ug

目录价：￥6127.00

市场价格：￥5207.95

会员价格：￥4901.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-acetyl-phospho Histone H1.4/H3 (Lys26/Lys9, Ser27/Ser10) Antibody | ABE375 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: Specificity was evaluated by dot blot analysis against a panel of peptides corresponding to various histone modification sites. Due to conservation of the ARKS motif, this antibody recognizes the N-terminus of Histone H1.4 acetylated at Lys26 and phosphorylated at Ser27, as well as Histone H3 acetylated at Lys9 and phosphorylated at Ser10. In addition, this antibody recognizes the N-terminus of Histone H1.4 acetylated at Lys26, and Histone H3 acetylated at Lys9. Dot blot specificity analysis did not detect any cross reactivity to acetylated Lys27 of Histone H3. View All» Molecular Weight: ~17 kDa observed View All» Epitope: N-terminus View All» Immunogen: KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H3 acetylated at Lys9 and phosphorylated at Ser10. View All» Background Information: Histones are nuclear proteins that form octameric structures, which bind DNA to form units of chromatin called nucleosomes. The family of histones H1, H2A, H2B, H3, and H4 are key players in gene regulation. Histones undergo a number of post-translational modifications (PTMs) in response to various stimuli including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes, depending on the cellular context. Often, it is the pattern of different modifications on several histone residues that alter chromatin, rather than modification of single residues. Histone H3 is observed to form the core octameric structure that is required for the packaging of DNA into nucleosomes, the most basic level of chromatin arrangement. H1.4 is one of four variants of Histone H1 (H1.2-H1.5) that can be found in somatic cells. The H1 Histones may function as linker proteins binding DNA via their GH domain, and enabling the wrapping of DNA around the core nucleosomal structure formed from the association of H2A, H2B, H3, and H4. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Demonstrated to react with Human, Rat, and Mouse. Predicted to demonstrate broad species reactivity. View All» Application Notes: Dot Blot (Specificity) Analysis: A representative lot detected Histone H1.4 acetylated at Lys26, and Histone H3 acetylated at Lys9. This antibody also detected Histone H1.4 acetylated at Lys26 and phosphorylated at Ser27, and Histone H3 acetylated at Lys9 and phosphorylated at Ser10.Immunoprecipitation Analysis: 1 µg from a representative lot immunoprecipitated acetyl-phospho Histone H1.4/H3 (Lys26/Lys9, Ser27/Ser10) in 500 µl of colcemid treated HeLa acid extract. View All» Control: Untreated and sodium butyrate treated HeLa acid extract, and recombinant Histone H3 protein View All» Quality Assurance: Evaluated by Western Blot in untreated and sodium butyrate treated HeLa acid extract and in recombinant Histone H3 protein.Western Blot Analysis: 0.5 µg/mL of this antibody detected acetyl-phospho Histone H3 (Lys9/Ser10) in 10 µg of untreated and sodium butyrate treated HeLa acid extract. Note that acetyl-phospho Histone H1.4 Lys27/Ser27 is not present in acid extracts and thus not detected in this blot. View All» Purification Method: Affinity purified View All» Presentation: Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: P68431 View All» Entrez Gene Number: NP_003520 View All» Gene Symbol:
  • HIST1H3A

  • H3FA

  • HIST1H3B

  • H3FL

  • HIST1H3C

  • H3FC

  • HIST1H3D

  • H3FB

  • HIST1H3E

  • H3FD

  • HIST1H3F

  • H3FI

  • HIST1H3G

  • H3FH

  • HIST1H3H

  • H3FK

  • HIST1H3I

  • H3FF
    View All» Alternate Names:
    • Histone H3.1

    • Histone H3/a

    • Histone H3/b

    • Histone H3/c

    • Histone H3/d

    • Histone H3/f

    • Histone H3/h

    • Histone H3/I

    • Histone H3/j

    • Histone H3/k

    • Histone H3/l
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Dot Blot

      • Immunoprecipitation

      • Western Blotting
        View All» Product Name: Anti-acetyl-phospho Histone H1.4/H3 (Lys26/Lys9, Ser27/Ser10) View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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