货号：ABE42

品牌：Merck Millipore

规格：100Ug

目录价：￥3789.00

市场价格：￥3220.65

会员价格：￥3031.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-TAF(II)250 Antibody | ABE42 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes TAF(II)250 at the protein kinase 2 domain. View All» Molecular Weight: ~ 225 kDa observed. Uncharacterized bands may be observed between ~70 and 220 kDa in some lysates. View All» Epitope: Protein kinase 2 domain View All» Immunogen: KLH-conjugated linear peptide corresponding to human TAF(II)250 at the protein kinase 2 domain. View All» Background Information: TAF(II)250, also referred to as TAF1, is an N-terminal kinase and the largest subunit of the human transcription factor TFIID. TAF(II)250 includes serine/threonine kinase domain that has the ability to transphosphorylate and autophosphorylate the basal factor TFIIF large subunit. TAF(II)250 has been shown to form a complex with B-Myb which activated Myb responsive promoters and plays a role in cell cycle regulation. TAF(II)250 is also important in the preservation of Mdm2 phosphorylation and function, which negatively regulates the p53 tumor suppressor protein. View All» Species Reactivity:

• Human

• Mouse

• Rat

• Bovine

• Horse

• Canine
  View All» Species Reactivity Note: Demonstrated to react with Human. Predicted to react with Mouse, Rat, Bovine, Equine, and Canine based on 100% sequence homology. View All» Control: HepG2 cell lysate View All» Quality Assurance: Evaluated by Western Blot in HepG2 cell lysate.Western Blot Analysis: 1 µg/mL of this antibody detected TAF(II)250 on 10 µg of HepG2 cell lysate. View All» Purification Method: Affinity purified View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: P21675 View All» Entrez Gene Number: NP_004597 View All» Gene Symbol:
  • TAF1

  • BA2R

  • CCG1

  • CCGS

  • TAF2A
    View All» Alternate Names:
    • Transcription initiation factor TFIID subunit 1

    • Cell cycle gene 1 protein

    • TBP-associated factor 250 kDa

    • p250

    • Transcription initiation factor TFIID 250 kDa subunit

    • TAF(II)250

    • TAFII-250

    • TAFII250
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. This gene is part of a complex transcriptional unit (TAF1/DYT3), wherein some products share exons with TAF1 as well as additional exons downstream.[provided by RefSeq]. View All» UniProt Summary: FUNCTION: Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.COFACTOR: Magnesium.ENZYME REGULATION: Autophosphorylates on Ser residues. Inhibited by retinoblastoma tumor suppressor protein, RB1.SUBUNIT STRUCTURE: TAF1 is the largest component of transcription factor TFIID that is composed of TBP and a variety of TBP-associated factors. TAF1, when part of the TFIID complex, interacts with C-terminus of TP53. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, IN80C, KIAA1267, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. RB1 interacts with the N-terminal domain of TAF1. Interacts with ASF1A and ASF1B. Interacts with SV40 Large T antigen. SUBCELLULAR LOCATION: Nucleus PTM: Phosphorylated by casein kinase II in vitro. INVOLVEMENT IN DISEASE: Defects in TAF1 are the cause of dystonia type 3 (DYT3) [MIM:314250]; also called X-linked dystonia-parkinsonism (XDP). DYT3 is a X-linked dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT3 is characterized by severe progressive torsion dystonia followed by parkinsonism. Its prevalence is high in the Philippines. DYT3 has a well-defined pathology of extensive neuronal loss and mosaic gliosis in the striatum (caudate nucleus and putamen) which appears to resemble that in Huntington disease. SEQUENCE SIMILARITIES: Belongs to the TAF1 family.Contains 2 bromo domains.Contains 1 HMG box DNA-binding domain.Contains 2 protein kinase domains. View All» Product Name: Anti-TAF(II)250 View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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