货号：ABE435

品牌：Merck Millipore

规格：200UL

目录价：￥6127.00

市场价格：￥5207.95

会员价格：￥4901.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-trimethyl-Histone H3 (Lys36) Antibody | ABE435 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes Histone H3 trimethylated at Lys36. View All» Molecular Weight: ~17 kDa observed. An uncharacterized band appears at ~52 kDa in some lysates. View All» Epitope: Trimethylated Lys36 View All» Immunogen: KLH-conjugated linear peptide corresponding to human Histone H3 trimethylated at Lys36. View All» Background Information: Histones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Methylation occurs on histone H3 at lysine 4, 9, 27, 36, and 79 residues. Previous studies have reported that methylation of H3 lysine 9 provides a binding site for heterochromatin adaptor proteins (HP1) which are involved in the formation of heterochromatin and gene repression. Phosphorylation of histones may also produce similar repressive effects depending on the cellular context. Often, it is the pattern of different modifications on several histone residues that alter chromatin, rather than modification of single residues. View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Demonstrated to react with Human and Mouse. Broad species reactivity expected due to similar sequence homology. View All» Application Notes: Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see Table) were probed with Anti-trimethyl-Histone H3 (Lys36) (1:2,000 dilution).Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Histone H3 in A431 cells.ChIP-Sequencing: Chromatin immunoprecipitation was performed using the Magna ChIP™ HiSens kit (cat#17-10460, 2 µg of anti-trimethyl-Histone H3 (Lys36) antibody(ABE435), 20 µL Protein A/G beads , and 1e6 crosslinked HeLa cell chromatin followed by DNA purification using magnetic beads. Libraries were prepared from Input and ChIP DNA samples using standard protocols with Illumina barcoded adapters, and analyzed on Illumina HiSeq™ instrument. An excess of eleven million reads from FastQ files were mapped using Bowtie (http://bowtie-bio.sourceforge.net/manual.shtml) following TagDust (http://genome.gsc.riken.jp/osc/english/dataresource/) tag removal. Peaks were identified using MACS (http://luelab.dfci.harvard.edu/MACS/), with peaks and reads visualized as a custom track in UCSC Genome Browser (http://genome.ucsc.edu) from BigWig and BED files. View All» Control: HeLa cell extract and recombinant Histone H3 protein View All» Quality Assurance: Evaluated by Western Blot in HeLa cell extract and recombinant Histone H3 protein.Western Blot Analysis: A 1:500 dilution of this antibody detected Histone H3 on 10 µg of HeLa cell extract and recombinant Histone H3 protein. View All» Purification Method: Affinity purified View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q16695 View All» Entrez Gene Number: NP_003484 View All» Gene Symbol:
  • HIST1H3A

  • H3FA

  • HIST1H3B

  • H3FL

  • HIST1H3C

  • H3FC

  • HIST1H3D

  • H3FB

  • HIST1H3E

  • HIST1H3F

  • H3FI

  • HIST1H3G

  • H3FH

  • HIST1H3H

  • H3FK

  • HIST1H3I

  • H3FF

  • HIST1H3J

  • H3FJ
    View All» Alternate Names:
    • Histone H3.1

    • Histone H3/a

    • Histone H3/b

    • Histone H3/c

    • Histone H3/d

    • Histone H3/f

    • Histone H3/h

    • Histone H3/I

    • Histone H3/j

    • Histone H3/k

    • Histone H3/l
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunocytochemistry

      • Dot Blot

      • Chromatin Immunoprecipitation (ChIP)

      • ChIP-seq
        View All» Product Name: Anti-trimethyl-Histone H3 (Lys36) View All» Concentration: 0.25 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 200 µL View All» Format: Affinity Purified View All» Host: Rabbit View All»
        © 金山科研平台是专业的授权总代理区域代理经销平台。
        © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
        © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
        © 本文地址：https://www.16ao.com/thread-159094.htm
        © Merck Millipore代理ABE435 Anti-trimethyl-Histone H3 (Lys36) Antibody;抗-三甲基-组蛋白 H3（Lys36）抗体store at +2℃ to +8℃，产品报价联系微信jinshanbio