货号：ABE78

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Aly/REF Antibody | ABE78 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Molecular Weight: ~32 kDa observed. An uncharacterized band at ~68 kDa may be observed in some lysates. View All» Epitope: Unknown View All» Immunogen: Recombinant protein corresponding to human Aly/REF. View All» Isotype: IgG View All» Background Information: Aly (THO complex subunit 4 or THOC4 or Aly/REF) is a heat stable, nuclear protein that functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins. Aly (THOC4) is also preferentially associated with intronless mRNAs in the nucleus. Loading of export factors onto mRNAs is a key step in gene expression. In vertebrates, splicing plays a role in this process. Specific protein complexes, exon junction complexes and transcription/export complexes, are loaded onto mRNAs in a splicing-dependent manner, and adaptor proteins such as Aly (THOC4) in the complexes in turn recruit mRNA exporters onto the RNA. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Demonstrated to react with human, mouse, and rat. View All» Application Notes: Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Aly/REF in HeLa cells. View All» Control: HeLa cell lysate View All» Quality Assurance: Evaluated by Western Blot in HeLa cell lysate.Western Blot Analysis: A 1:1,000 dilution of this antibody detected Aly/REF in 10 µg of HeLa cell lysate. View All» Purification Method: Unpurified View All» Presentation: Rabbit polyclonal serum containing 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: Q86V81 View All» Entrez Gene Number: NP_005773 View All» Gene Symbol:
  • ALY

  • BEF

  • Tho4

  • THOC4
    View All» Alternate Names:
    • Ally of AML-1 and LEF-1

    • THO complex 4

    • Transcriptional coactivator Aly/REF

    • bZIP enhancing factor

    • bZIP-enhancing factor BEF

    • transcriptional coactivator
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunocytochemistry
        View All» Entrez Gene Summary: The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Acts as chaperone and promotes the dimerization of transcription factors containing basic leucine zipper (bZIP) domains and thereby promotes transcriptional activation. Plays a role in mRNA processing and export. May function as scaffold that mediates interactions between proteins and/or RNA. Integral part of the THO/TREX complex that is recruited to transcribed genes and travels with the RNA polymerase during elongation. Is part of the exon junction complex that remains associated with spliced mRNA and plays an important role in mRNA export and nonsense-mediated RNA decay. Directs mRNA derived from Herpes simplex virus intron-less genes to the NXF1-mediated export pathway.SUBUNIT STRUCTURE: Homomultimer. Binds LEF1 and RUNX1 By similarity. Is part of several complexes involved in mRNA processing and export. Part of the heteromultimeric THO/TREX complex containing THOC1, THOC2, THOC3, THOC4 and UAP56. Associates with the spliceosome. Binds NXF1 and RBM8A directly and is part of the exon junction complex (EJC) containing NCBP1, NCBP2, RBM8A, SRRM1, NXF1, RENT2, RENT3B and THOC4 By similarity. Found in a mRNA splicing-dependent exon junction complex (EJC) with DEK, RBM8A, RNPS1, SRRM1 and THOC4. Binds UL54 from Herpes simplex type 1 (strain KOS). Found in a mRNP complex with RENT3A and RENT3B. Interacts with BAT1, RBM8A, RNPS1 and SRRM1. Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8.SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. PTM: Arg-50 and Arg-204 are dimethylated, probably to asymmetric dimethylarginine. Phosphorylated upon DNA damage, probably by ATM or ATR. INVOLVEMENT IN DISEASE: Antibodies against THOC4 are found in sera of patients with systemic lupus erythematosus (SLE). SEQUENCE SIMILARITIES: Contains 1 RRM (RNA recognition motif) domain. View All» Product Name: Anti-Aly/REF View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Serum View All» Host: Rabbit View All»
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