货号：ABF10

品牌：Merck Millipore

规格：EA

目录价：￥3108.00

市场价格：￥2641.80

会员价格：￥2486.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-RIG-I (Retinoic Acid Inducible RNA helicase) Antibody | ABF10 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes RIG-I. View All» Molecular Weight: ~ 110 kDa observed. View All» Immunogen: GST-tagged recombinant protein corresponding to mouse RIG-I. View All» Background Information: Retinoic acid-inducible gene 1 protein (RIG-1) is a cytoplasmic DExD/H box RNA helicase belonging to the helicase family and containing 2 CARD domains within the N-terminus, a repressor domain (RD), a helicase ATP-binding domain and a helicase C-terminal binding domain. RIG-1 is able to bind the 5’-triphosphate RNA of single or double-stranded viral RNA. After recognizing and binding viral RNA, RIG-1 protein initializes type I IFN production, and within fibroblasts and dendritic cells plays a central role in the regulation of type I IFN production in response to viral presence. There are four known isoforms resulting from alternative splice variants. View All» Species Reactivity: Mouse View All» Species Reactivity Note: Demonstrated to react with mouse. View All» Application Notes: Western Blot Analysis: A 1:1,000 dilution of this antibody detected RIG-I in 10 µg of IFN-β-treated RAW264.7 cell lysates. View All» Control: Untreated and IFN-β-treated MEF cell lysates View All» Quality Assurance: Evaluated by Western Blot in untreated and IFN-β-treated MEF cell lysates.Western Blot Analysis: A 1:1,000 dilution of this antibody detected RIG-I in 10 µg of IFN-β-treated MEF cell lysates. View All» Purification Method: Unpurified View All» Presentation: Rabbit polyclonal serum containing 0.05% sodium azide View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: Q6Q899 View All» Entrez Gene Number: NP_766277 View All» Gene Symbol:

• RIG-I

• RIG-1
  View All» Alternate Names:
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 58

  • retinoic acid inducible gene I

  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide

  • retinoic acid-inducible gene I

  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide RIG-I

  • DEAD-box protein 58

  • RNA helicase RIG-I

  • Retinoic acid-inducible gene 1 protein
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» UniProt Summary: FUNCTION: Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III. Essential for the production of interferons in response to RNA viruses including paramyxoviruses, and possibly other negative strand RNA viruses. COFACTOR: Zinc (By similarity).SUBUNIT STRUCTURE: Monomer; maintained as a monomer in an autoinhibited state. Upon viral dsRNA binding and conformation shift, homomultimerizes and interacts with MAVS. Interacts with DHX58/LGP2, IKBKE, TBK1 and TMEM173/STING (By similarity). Interacts (via CARD domain) with TRIM25 (via SPRY domain) (By similarity).SUBCELLULAR LOCATION: Cytoplasm (By similarity). Note: Colocalized with TRIM25 at cytoplasmic perinuclear bodies (By similarity).DOMAIN: The helicase domain is responsible for dsRNA recognition (By similarity).The 2 CARD domains are responsible for interaction with and signaling through MAVS (By similarity).The repressor domain controls homomultimerization and interaction with MAVS (By similarity).The second CARD domain is the primary site for 'Lys-63'-linked ubiquitination (By similarity).PTM: Isgylated. Conjugated to ubiquitin-like protein ISG15 upon IFN-beta stimulation (By similarity).Ubiquitinated (By similarity).DISRUPTION PHENOTYPE: Death between E12.5 and E14 due to liver apoptosis. Those who are born alive show growth retardation and die within 3 weeks. SEQUENCE SIMILARITIES: Belongs to the helicase family.Contains 2 CARD domains.Contains 1 helicase ATP-binding domain.Contains 1 helicase C-terminal domain. View All» Product Name: Anti-RIG-I (Retinoic Acid Inducible RNA helicase) View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Serum View All» Host: Rabbit View All»
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