货号：ABN22

品牌：Merck Millipore

规格：

目录价：￥4146.00

市场价格：￥3524.10

会员价格：￥3316.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-UCH-L1 Antibody | ABN22 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Molecular Weight: ~26 kDa observed View All» Immunogen: Histidine-tagged recombinant protein corresponding to human UCH-L1. View All» Background Information: Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1), a thiol protease belonging to the peptidase C12 family, is involved in the processing of ubiquitin precursors and of ubiquitinated proteins. UCH-L1 recognizes and hydrolyzes peptide bonds at the C-termial glycine of ubiquitin. UCH-L1 is expressed in neurons and cells of the diffuse neuroendocrine system and thier tumors. Defects in UCH-L1 are the cause of Parkinson disease type 5. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Demonstrated to react with Human, Mouse, and Rat. View All» Control: PC12 cell lysate View All» Quality Assurance: Evaluated by Western Blot in PC12 cell lysate.Western Blot Analysis: 0.2 µg/mL of this antibody detected UCH-L1 on 10 µg of PC12 cell lysate. View All» Purification Method: IgY purified View All» Presentation: Purified chicken polyclonal in PBS containing 0.05% sodium azide and 10% glycerol. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P09936 View All» Entrez Gene Number: NP_004172 View All» Gene Symbol: UCHL1 View All» Alternate Names:
  • Ubiquitin carboxyl-terminal hydrolase isozyme L1

  • UCH-L1

  • Neuron cytoplasmic protein 9.5

  • PGP 9.5

  • PGP9.5

  • Ubiquitin thioesterase L1
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease. View All» UniProt Summary: FUNCTION: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.CATALYTIC ACTIVITY: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). SUBUNIT STRUCTURE: Monomer. Homodimer. Interacts with SNCA By similarity. Interacts with COPS5. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. PTM: O-glycosylated (By similarity).INVOLVEMENT IN DISEASE: Defects in UCHL1 are the cause of Parkinson disease type 5 (PARK5) [MIM:613643]; also known as Parkinson disease autosomal dominant 5. PARK5 is a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Clinical features include parkinsonian symptoms (resting tremor, rigidity, postural instability and bradykinesia), dementia, diffuse Lewy body pathology, autonomic dysfunction, hallucinations and paranoia. MISCELLANEOUS: Oxidation of Met-1, Met-6, Met-12, Met-124 and Met-179 to methionine sulfoxide, and oxidation of Cys-220 to cysteine sulfonic acid have been observed in brains from Alzheimer disease (AD) and Parkinson disease (PD) patients. In AD, UCHL1 was found to be associated with neurofibrillary tangles. In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8.SEQUENCE SIIMILARITIES: Belongs to the peptidase C12 family.Caution Ref.8 reports the association of mutation Ile93Met with Parkinson disease. However, according to Ref.26 this association is uncertain and UCHL1 is not a susceptibility gene for Parkinson disease.BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters:KM=122 nM for Ub-AMC KM=1.20 µM for ubiquitin ethyl esterVmax=0.47 µmol/min/mg enzyme toward Ub-AMCVmax=25 µmol/min/mg enzyme toward ubiquitin ethyl esterSEQUENCE CAUTION: The sequence CAA28443.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. View All» Product Name: Anti-UCH-L1 View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Chicken View All»
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