货号：ABS108

品牌：Merck Millipore

规格：

目录价：￥3346.00

市场价格：￥2844.10

会员价格：￥2676.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-LRRK2 (Tyr1491) Antibody | ABS108 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes the ROC domain of LRRK2 phosphorylated at Tyr1491. View All» Molecular Weight: ~200 kDa observed. The observed MW is the truncated wild-type LRRK2 transfected protein. The calculated molecular weight of endogenous LRRK2 is ~286 kDa. View All» Epitope: ROC domain View All» Immunogen: KLH-conjugated linear peptide corresponding to the ROC domain of human LRRK2 phosphorylated at Tyr1491. View All» Modifications: Phosphorylation View All» Background Information: Leucine-rich repeat kinase 2 (LRRK2) is a 2,527 amino acid protein encoded by the PARK8 gene and belonging to the ROCO family of proteins. This protein family is characterized as having a unique interaction between the Roc domain and the intrinsic GTPase activity along with a COR domain. LRKK2 consists of 5 different domains; a tyrosine kinase-like MAPKKK-related domain, WD40 propeller motif repeats, a Roc (Ras-like small GTPase family) domain, a COR (C-terminal of Roc) domain, and an LRRK. LRRK2 is mainly localized to the cytoplasm and expression within tissue is most highly observed within the brain. It is found in lower levels within the liver, heart, lung, skeletal muscles, placenta, pancreas, and kidney. The exact function of LRRK2 is unknown; however mutations to any of the five domains are the most commonly known causes of Parkinson’s disease, both familial and sporadic. Further study of this protein may lead to a better understanding of the disease’s pathogenesis, as well as potential therapeutic markers. View All» Species Reactivity:

• Human

• Mouse

• Rat

• Pig

• Baboon

• Bovine
  View All» Species Reactivity Note: Demonstrated to react with Human. Predicted to react with Mouse, Rat, Porcine, Baboon, and Bovine based on 100% sequence homology. View All» Application Notes: Peptide Inhibition Analysis: 0.2 µg/mL from representative lot blocked phospho-LRRK2 (Tyr1491) in cell lysates transfected with truncated wild-type LRRK2. View All» Control: Cell lysates transfected with truncated forms of wild-type LRRK2 with auto-phosphorylation properties and mutant D1994A LRRK2 which lacks auto-phosphorylation properties. View All» Quality Assurance: Evaluated by Western Blot in lysates from cells transfected with truncated forms of wild-type LRRK2 and mutant D1994A LRRK2. D1994A LRRK2 contains a mutation at aspartic acid 1994 which limits its auto-phosphorylation activity.Western Blot Analysis: 0.2 µg/mL of this antibody detected phospho-LRRK2 (Tyr1491) in lysates from cells transfected with truncated wild-type LRRK2, but not in lysates from cells transfected with mutant D1994A LRRK2. View All» Purification Method: Affinity purified View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q5S007 View All» Entrez Gene Number: NP_940980 View All» Gene Symbol:
  • LRRK2

  • PARK8
    View All» Alternate Names:
    • Leucine-rich repeat serine/threonine-protein kinase 2

    • Dardarin
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Peptide Inhibition Assay
        View All» Entrez Gene Summary: This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. View All» UniProt Summary: FUNCTION: Probable protein kinase whose role is not yet known. May play a role in the phosphorylation of proteins central to Parkinson disease. May also have GTPase activity.CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.SUBUNIT STRUCTURE: Interacts with PARK2.SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Note: Localized in the cytoplasm and associated with cellular membrane structures. Associates with the mitochondrial outer membrane.TISSUE SPECIFICITY: Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas.INVOLVMENT IN DISEASE: Defects in LRRK2 are the cause of Parkinson disease type 8 (PARK8). A slowly progressive neurodegenerative disorder characterized by bradykinesia, rigidity, resting tremor, postural instability, neuronal loss in the substantia nigra, and the presence of neurofibrillary MAPT (tau)-positive and Lewy bodies in some patients. View All» Product Name: Anti-phospho-LRRK2 (Tyr1491) View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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