货号:ABS375
品牌:Merck Millipore
规格:
目录价:¥3475.00
市场价格:¥2953.75
会员价格:¥2780.00
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Description:
Anti-DKK1
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Promotional Text:
Special Shipping Offer on Antibodies100% Performance Guaranteed
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Molecular Weight:
~44 kDa observed. An uncharacterized band may appear at ~17 kDa in some lysates. The calculated molecular weight is 29 kDa, however DKK1 has been shown as a ~44 kDa band in western blots (Binnerts, M.E., et al. (2006). PNAS. 104(37):14700-14705.)
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Immunogen:
GST-tagged recombinant protein corresponding to human DKK1.
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Background Information:
DKK1, also named a SK and Dickkopf-1, belongs to the dickkopf family. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, DKKs are implicated in bone formation and bone disease, cancer and Alzheimer's disease.
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Species Reactivity:
Human
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Species Reactivity Note:
Demonstrated to react with Human.
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Application Notes:
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected DKK1 in human placenta and prostate cancer tissues.Immunofluorescence Analysis: A 1:25 dilution from a representative lot detected DKK1 in HepG2 cells.
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Control:
MCF-7 cell lysate
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Quality Assurance:
Evaluated by Western Blotting in MCF-7 cell lysate.Western Blotting Analysis: A 1:500 dilution of this antibody detected DKK1 in 10 µg of MCF-7 cell lysate.
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Purification Method:
Antigen Affinity purified
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Presentation:
Purified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide and 50% glycerol.
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Storage Conditions:
Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
O94907
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Gene Symbol:
DKK1
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Alternate Names:
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DKK1
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Dickkopf 1
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Dickkopf related protein 1
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DKK 1
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SK
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hDkk 1
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
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Western Blotting
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Immunohistochemistry
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Immunofluorescence
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Product Name:
Anti-DKK1
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Concentration:
0.24 mg/mL
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Antibody Type:
Polyclonal Antibody
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Qty/Pk:
150 µL
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Format:
Affinity Purified
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Host:
Rabbit
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