货号：ABS63

品牌：Merck Millipore

规格：EA

目录价：￥3024.00

市场价格：￥2570.40

会员价格：￥2419.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-USP33 Antibody | ABS63 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Molecular Weight: ~ 110 kDa observed View All» Epitope: Unknown View All» Immunogen: GST-tagged recombinant protein corresponding to human USP33. View All» Background Information: Ubiquitin-specific-processing protease 33 (USP33) is a widely expressed, deubiquitinating enzyme first identified as a von Hippel-Lindau tumor suppressor (VHL) protein-interacting deubiquitinating enzyme (hVDU1) and is a member of the peptidase C19 family. USP33 is involved in a variety of processes including beta-2 adrenergic receptor/ADRB2 recycling and cell migration. Known to bind Robo1 receptor, USP33 is a required component of the Slit signaling pathway responsiveness in breast cancer cells. Slit redistributes Robo1 to the plasma membrane from intracellular compartments in a manner dependent on the presence UP33, which produces an inhibitory effect on breast cancer cell directional migration. USP33 has been shown to bind to and deubiquitinate D2 as a crucial response to cold exposure, helping to decrease D2 degradation, and acclimatize brown adipocytes. It also mediates the beta-arrestin and beta-2 adrenergic receptor deubiquitination, thereby regulating GPCR signaling. View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Demonstrated to react with human and mouse. View All» Application Notes: Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected USP33 in A431 cells. View All» Control: HeLa cell lysate View All» Quality Assurance: Evaluated by Western Blot in HeLa cell lysate.Western Blot Analysis: 1;1,000 dilution of this antibody detected USP33 on 10 µg of HeLa cell lysate. View All» Purification Method: Affinity purified View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q8TEY7 View All» Entrez Gene Number: NP_055832 View All» Gene Symbol:
  • USP33

  • hVDU1

  • VDU1
    View All» Alternate Names:
    • ubiquitin specific peptidase 33

    • VHL-interacting deubiquitinating enzyme 1

    • pVHL-interacting deubiquitinating enzyme 1

    • ubiquitin specific protease 33

    • ubiquitin carboxyl-terminal hydrolase 33

    • Ubiquitin thioesterase 33

    • Ubiquitin-specific-processing protease 33

    • Deubiquitinating enzyme 33
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunocytochemistry
        View All» UniProt Summary: FUNCTION: Deubiquitinating enzyme involved in various processes such as cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. CATALYTIC ACTIVITY: Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol.SUBUNIT STRUCTURE: Interacts with VHL, leading to its ubiquitination and subsequent degradation. Interacts with ARRB1, ARRB2, ADRB2, DIO2 and ROBO1. Interacts with SELENBP1; in a selenium-dependent manner. SUBCELLULAR LOCATION: Cytoplasm › perinuclear region. Note: According to Ref.5, it localizes in the endoplasmic reticulum; however the relevance of such result is unclear. TISSUE SPECIFICTY: Widely expressed. DOMAIN: The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 86 is replaced by a Glu residue. PTM: Ubiquitinated via a VHL-dependent pathway for proteasomal degradation. SEQUENCE SIMILARITIES: Belongs to the peptidase C19 family. USP20/USP33 subfamily.Contains 2 DUSP domains.Contains 1 UBP-type zinc finger.SEQUENCE CAUTION: The sequence BAA83049.1 differs from that shown. Reason: Erroneous initiation. View All» Product Name: Anti-USP33 View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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