货号：AG325

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Fluoro-Jade® C View All» Trade Name: Chemicon (Millipore) View All» Qty/Pk: 50 mg View All» Product Overview: Fluoro-Jade® C, like its predecessors, Fluoro-Jade® and Fluoro-Jade® B, were found to stain all degenerating neurons, regardless of specific insult or mechanism of cell death. Therefore, the patterns of neuronal degeneration seen following exposure to either the glutamate agonist, kainic acid, or the inhibitor of mitochondrial respiration, 3-NPA, were the same for all of the Fluoro-Jadeâ dyes. However, there was a qualitative difference in the staining characteristics of the three fluorochromes. Specifically, Fluoro-Jade® C exhibited the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localizing not only degenerating nerve cell bodies, but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols. Triple labeling can be accomplished by staining degenerating neurons with Fluoro-Jade® C, cell nuclei with DAPI and activated astrocytes with GFAP immunofluorescence. APPEARANCE: Coffee brown to brick red powder. MOLECULAR WEIGHT: 823 EXCITATION PEAK: 485 nm EMISSION PEAK: 525 nm FILTER SYSTEM: Fluorescein / FITC SOLUBILITY: Highly soluble in water and bases, moderately soluble in alcohol and weak acids. TOXICITY: Although the compound appears to be of low toxicity, it has not been extensively evaluated and therefore routine laboratory caution should be exercised. Not intended for human consumption. View All» Background Information: Fluoro-Jade C, like its predecessors, Fluoro-Jade and Fluoro-Jade B, were found to stain all degenerating neurons, regardless of specific insult or mechanism of cell death. Therefore, the patterns of neuronal degeneration seen following exposure to either the glutamate agonist, kainic acid, or the inhibitor of mitochondrial respiration, 3-NPA, were the same for all of the Fluoro-Jade dyes. However, there was a qualitative difference in the staining characteristics of the three fluorochromes. Specifically, Fluoro-Jade C exhibited the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localizing not only degenerating nerve cell bodies, but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols. Triple labeling can be accomplished by staining degenerating neurons with Fluoro-Jade C, cell nuclei with DAPI and activated astrocytes with GFAP immunofluorescence. Fluoro-Jade™ is a registered trademark of Histo-Chem, Inc. View All» Key Applications: Immunohistochemistry View All» Application Notes: SUGGESTED PROTOCOL FOR USING FLUORO-JADE® C Processing: Half of each group of brains were paraffin embedded and cut on a rotary microtome while the remainder were cut on a freezing sliding microtome. Paraffin sections were 10um in thickness while frozen sections were cut at a thickness of 25um. Prior to staining, sections were mounted from distilled water onto gelled slides. Gelatin coated slides were prepared by immersion in a 60 degree C solution of 1% pig skin gelatin (Sigma; type A, 300 Bloom) and then oven dried overnight at the same temperature. The sections were mounted onto the slides from distilled water and then air dried for at least 30 min on a slide warmer at 50 degrees C. Slides bearing frozen cut tissue sections were first immersed in a basic alcohol solution consisting of 1% sodium hydroxide in 80% ethanol for 5 min. They were then rinsed for 2 min in 70% ethanol, for 2 min in distilled water, and then incubated in 0.06% potassium permanganate solution for 10 min. Following a 1-2 min water rinse, the slides were then transferred for 10 min to a 0.0001% solution of Fluoro-Jade® C dissolved in 0.1% acetic acid vehicle. The proper dilution was accomplished by first making a 0.01% stock solution of the dye in distilled water and then adding 1 mL of the stock solution to 99 mL of 0.1% acetic acid vehicle. The working solution was used within 2 h of preparation. The stock solution, when refrigerated, can be kept for long periods but should be discarded if the solution becomes cloudy. The slides were then rinsed through three changes of distilled water for 1 min per change. Excess water was drained onto a paper towel, and the slides were then air dried on a slide warmer at 50 degrees C for at least 5 min. The air dried slides were then cleared in xylene for at least 1 min and then coverslipped with DPX (Fluka or Sigma) nonfluorescent mounting media. Polar coverslipping media, such as those that contain water, alcohol or glycerol were never used.For comparative purposes, some slides were stained with Fluoro-Jade® B according to the previously described procedure. When working with paraffin processed tissue, the sections are first deparaffinized through two 10 min changes of xylene and then the sections are rehydrated through a graduated alcohol series, omitting the basic alcohol solution. Once in distilled water, the sections are transferred to the potassium permanganate solution at which point the staining procedure is identical to that described for frozen sections. Multiple labeling: Fluoro-Jade® C can readily be combined with other fluorescent markers. Multiple labeling was achieved using anti-glial fibrillary acidic protein (GFAP) immunocytochemistry to label activated astrocytes while using DAPI to label nuclear DNA. Incorporating 4',6-diamidino-2-phenylindole (DAPI; Sigma, St. Louis MO) as a fluorescent nuclear stain is accomplished by simply incorporating 0.0001% into the Fluoro-Jade® C staining solution. This is accomplished by the addition of 1 ml of 0.01% DAPI stock solution to 99 ml of 0.1% acetic acid. Fluoro-Jade® C was also combined with immunofluorescent labeling of GFAP according to the following procedure. Loose frozen tissue sections were incubated in a prediluted solution of anti-GFAP (Chemicon has a number of different antibodies to GFAP) at about 5 degrees C in the refrigerator for 1-3 days. It should be mentioned that although in this study all immunocytochemistry was performed on frozen sections, the methods are fully compatible with paraffin processed tissue as well. Sections were rinsed in two changes of buffered saline for 10 min each and then transferred to a tetramethylrhodamine isothiocynate (TRITC) labeled secondary antibody (Chemicon has a number of different TRITC labeled secondary antibodies), diluted 1:100 in buffered saline, for 1 h at room temperature. Sections were rinsed in two changes of buffered saline for 10 min each and then the sections were mounted onto gelled slides from distilled water and air dried on a slide warmer at 50 degrees C for 30 min. To combine with Fluoro-Jade® C, the slide mounted sections were rehydrated for 2 min in distilled water and then transferred to the 0.06% potassium permanganate solution for 10 min. It is worth mentioning that the incubation time in potassium permanganate may need to be reduced when co-localizing those antigenic epitopes susceptible to chemical oxidation. The slides were then rinsed for 2 min in distilled water, transferred to the Fluoro-Jade® C working solution for 10 min and then rinsed, air dehydrated, xylene cleared and coverslipped with DPX, as previously described. The blue nuclear label conferred by DAPI is visualized via ultraviolet light excitation, while the red TRITC labeled antibody is visualized by green light excitation. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Brand Family: Chemicon View All» Storage Conditions: The lyophilized powder should be stored well sealed at room temperature, preferable in a desiccator (due to its hygroscopic nature) for up to one year from date of receipt. The liquid stock solution (0.01%) in distilled water can be stored at 2-8°C for up to 3 months. The .0002-.0001% working solution in 0.1% acetic acid should be used within 4 hrs of preparation. View All» Promotional Text: This product has been replaced by AG325-30UGSpecial Shipping Offer on Antibodies100% Performance GuaranteedGet up to $500 Reward for Publishing View All» Product Name: Fluoro-Jade® C View All» Purity: Silica TLC (acetanitrile/water, 6/4) revealed the presence of two fluorescent spots, presumably corresponding to the di sulphate homologues. The presence of precursors or free fluorescein was not detected. View All»