产品英文名称：NS/0 HCP ELISA kit

产品中文名称：NS/0 HCP ELISA 试剂盒

NS/0 HCP ELISA kit 的应用

本试剂盒是用于确定在NS/0 的重组表达产品细胞中NS/0 宿主细胞蛋白污染的存在。该试剂盒是用于研究和制造使用，不用于人类或动物的诊断应用。

NS/0 HCP ELISA 试剂盒的英文描述

Hybridization of antibody producing cells with NS/0 cells is one of the most commonly employed systems for the production of humanized and mouse monoclonal antibodies. This kit has been successfully qualified for a number of different therapeutic human monoclonal antibodies. Our assay validation has determined that the LOD for this kit is ~300pg/mL.

NS/0 HCP ELISA kit 的使用步骤规则

The NS/0 assay is a two-site immunoenzymetric assay. Samples containing NS/0 HCPs are reacted with a horseradish peroxidase (HRP) enzyme labeled anti-NS/0 antibody in a test tube or simultaneously in microtiter strips coated with an affinity purified capture anti-NS/0 antibody. The immunological reactions result in the formation of a sandwich complex of solid phase antibody-HCP-enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethyl benzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of NS/0 HCPs present.

NS/0 HCP ELISA 试剂盒的保存和稳定性

1 All reagents should be stored at 2℃ to 8C℃for stability until the expiration date printed.

2 The substrate reagent should not be used if its stopped absorbance at 450nm is greater than 0.1.

3 Reconstituted wash solution is stable until the expiration date of the kit.

NS/0 HCP ELISA 试剂盒的使用注意事项

1 只用于研究或制造。

2 停止反应试剂是0.5N 硫酸。避免接触眼睛，皮肤，和服装

3 本试剂盒所用的标准包含来自人血清免疫球蛋白。源材料进行了测试，发现有艾滋病和乙型肝炎表面抗原的抗体。没有任何已知的方法可以使HIV或肝炎病毒或其他病原体总保证提供，因此处理这些试剂，好像他们是潜在的传染。在用本试剂盒的试剂浓度，其他没有被认为是有害的。

4 本试剂盒只能由合格的技术人员使用。