货号：AMP3800

品牌：Merck Millipore

规格：EA

目录价：￥4500.00

市场价格：￥3825.00

会员价格：￥3600.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Amplifluor® ID Herpes Virus Type 1 (HSV-1) & Type 2 (HSV-2) Kit View All» Trade Name:

• Amplifluor

• Chemicon (Millipore)
  View All» Qty/Pk: 100 assays View All» Product Overview: Test Principle: The Amplifluor® ID Human Herpesvirus Type 1 (HSV-1) and Type 2 (HSV-2) Kit was designed to detect conserved nucleic acid regions contained within each virion type. Since Herpes viruses contains an DNA genomes, Polymerase Chain Reaction (PCR) amplification utilizes the forward Amplifluor® hairpin primer and reverse primer to produce target-specific fluorescent amplicons. The Amplifluor® hairpin primer is labeled with Donor (fluorescein) and Quencher (DABSYL) fluorophores, which produce the high signal to noise ratio observed in the Amplifluor® assay. Upon incorporation into new amplicons, the Amplifluor® primer is fluorescent (reported in the instrument's FAM layer), while the fluorescein signal of the unincorporated Amplifluor® primer is quenched. When performed on a thermal cycler capable of real-time fluorescence detection, the amplification products of the PCR reaction can be monitored directly in a quantitative fashion, allowing evaluation of template detection in a single, closed-tube system with no additional assay procedure. Where required, template standard curves may be employed to quantitatively determine viral load. View All» Key Applications: DNA Amplification View All» Application Notes: The Amplifluor® ID Herpes Virus Type 1 (HSV-1) & Type 2 (HSV-2) Kit is intended for the quantitative or qualitative evaluation of Herpes Type 1 or Type 2 Virus RNA in separate reactions.For Research Use Only View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Components:
  • 25X Amplifluor® ID HSV-1 Forward Primer, 100 reactions. Light sensitive, Store at or below -20°C.

  • 25X Amplifluor® ID HSV-1 Reverse Primer, 100 reactions. Store at or below -20°C.

  • 25X Amplifluor® ID HSV-2 Forward Primer, 100 reactions. Light sensitive, Store at or below -20°C.

  • 25X Amplifluor® ID HSV-2 Reverse Primer, 100 reactions. Store at or below -20°C.

  • Positive Control for HSV Type 1quantitation, 100 reactions. Store at or below -20°C.

  • Positive Control for HSV Type 2 quantitation, 100 reactions. Store at or below -20°C.
    View All» Brand Family: Chemicon View All» Storage Conditions: The Amplifluor® ID primers must be stored in the dark at -20ºC (or at -80ºC for long term storage). Amplifluor® primers are light sensitive. Amplifluor® primers may be divided into convenient aliquots and wrapped in foil to minimize light exposure. Warnings and Precautions: • Handle all specimens and materials coming in contact with them as potentially infectious materials. Decontaminate with 0.05% sodium hypochlorite (1:100 dilution of household bleach). • Do not use reagents beyond expiration date. • Nucleic acids are subject to degradation by nucleases found in the environment and on human surfaces. Clean and cover work surfaces with disposable coverings and wear powder free gloves during all procedures. • Use pipetting techniques that will deliver correct volumes of reagents in all steps, including pre-wetting aerosol resistant pipette tips. Repeating pipettors should be primed and voided of all air bubbles. • With nucleic acid amplification, the potential for carry-over contamination is high. Work in a unidirectional flow from workstation to workstation and always use dedicated equipment and aerosol-barrier pipette tips. View All» Product Name: Amplifluor® ID Herpes Virus Type 1 (HSV-1) & Type 2 (HSV-2) Kit View All» Materials Required but Not Delivered: 1. Nucleic Acid Extraction and Amplification Enzyme.2. Real-Time Instrument: ABI7500, Roche LC480 View All»
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