货号：AP340P-7ML

品牌：Merck Millipore

规格：EA

目录价：￥2030.00

市场价格：￥1725.50

会员价格：￥1624.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Goat anti-Mouse IgG Antibody, Poly-HRP, Ampli-HRP | AP340P-7ML View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Product Overview: Affinity purified goat anti mouse IgG, polymeric HRP linked antibody. View All» Background Information: Millipore's IHC Amplification reagent utilizes a novel controlled polymerization technology to prepare polymeric AP-linker antibody conjugates. The reagent is derived from small, multifunctional, polymerizable linkers that are used to activate a mixture of enzymes and linker antibodies, with polymerization under controlled conditions. Although the end product is a heterogeneous conjugate, with different molecular sizes, shapes and enzyme/antibody ratios, all results suggest that knowledge of the those parameters seems not to be critical with regard to performance. Compared to conventional biotin-streptavidin based three-step kits, the IHC Amplification reagent has the advantage of simpler and faster protocols, higher amplification power, biotin free and more consistent immunostaining outcomes on a variety of tissue types and antibodies (Shi et al. 1999). View All» Species Reactivity: Mouse View All» Application Notes: PROTOCOL: Immunohistochemistry: (All procedures should be performed at room temperature) · Deparaffinization, rehydration · Block endogenous peroxidase with 3% H2O2 in dd water for 10 minutes. · Perform antigen retrieval or enzyme digestion if necessary. 1. Block with blocking solution, 10 minutes. Blot off blocking solution. No need to wash. 2. Incubate the mouse primary antibody for 30-60 minutes, wash with PBS or TBS for 5 minutes, two times. (Incubation times with the primary antibody will need to be determined by the end user.) 3. Incubate with the ready-to-use Poly-HRP-Anti-Mouse IgG for 30 minutes, wash with PBS or TBS for 5 minutes, two times. 4. Incubate with DAB or AEC solution for 5 minutes, wash with dd water. 5. Counterstain with hematoxylin and mount. NOTES: 1. The system is biotin free so that blocking endogenous biotin is not necessary. 2. A pre-primary antibody blocking with 10% normal goat serum is recommended (CHEMICON catalog number S26-100ML). View All» Purification Method: Immunoaffinity purified View All» Presentation: Liquid. View All» Storage Conditions: Maintain product at 2-8°C for up to 12 months. WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:

• Immunohistochemistry

• Immunohistochemistry (Paraffin)
  View All» Brand Family: Chemicon View All» Product Name: Goat anti-Mouse IgG Antibody, Poly-HRP, Ampli-HRP | AP340P-7ML View All» Concentration: ready to use View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 7 mL View All» Format: HRP View All» Host: Goat View All»
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