货号：APT105

品牌：Merck Millipore

规格：EA

目录价：￥5253.00

市场价格：￥4465.05

会员价格：￥4202.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: CaspScreen™, non-adherent cells only View All» Trade Name:

• CaspSCREEN

• Chemicon (Millipore)
  View All» Qty/Pk: 25 assays View All» Product Overview: Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The CaspSCREEN™ Flow Cytometric Apoptosis Detection Kit provides a simple and convenient means for detecting activation of caspases by flow cytometry in intact cells. The assay utilizes a substrate that contains two aspartate residues linked to rhodamine 110 (D2R), a reported substrate for members of capase family proteases. The rhodamine 110 conjugated substrate is non-fluorescent, however, upon cleavage of the substrate by cellular caspases or apoptosis-related proteases, the released rhodamine 110 gives rise to fluorescence that can be measured at excitation of 488 nm and emission of 530 nm. As the D2R substrate is more cell-permeable than other fluorometric caspase substrates, apoptosis can easily be measured in intact cells by flow cytometry. View All» Key Applications:
  • Flow Cytometry

  • Activity Assay
    View All» Species Reactivity: Mammals View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Components:
    • D(2)R - 25 μL

    • DTT (1 M): 75 μL

    • Incubation Buffer: 7.5 mL
      View All» Brand Family: Chemicon View All» Storage Conditions: We recommend storing reagents at -20°C upon arrival. (Store Incubation Buffer at 4°C after opening). The performance of this product is guaranteed for 6 months. View All» Detection Methods: Fluorescent View All» Product Name: CaspScreen™, non-adherent cells only View All» Materials Required but Not Delivered: · Microcentrifuge and 1.5 mL Microcentrifuge tubes · 37°C water bath or incubator · Flow Cytometer View All»
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