货号：APT115

品牌：Merck Millipore

规格：

目录价：￥9633.00

市场价格：￥8188.05

会员价格：￥7706.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Apo-BrdU™ Flow Cytometry Detection Kit View All» Trade Name: Chemicon (Millipore) View All» Qty/Pk: 50 assays View All» Product Overview: The CHEMICON APO-BRDU™ Kit is a two color staining method for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry (Li et al. 1995). The kit contains the instructions and reagents required for measuring apoptosis in cells including; positive and negative control cells for assessing reagent performance; washing, reaction, and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), and fluorescein labeled anti-BrdU antibody for labeling DNA breaks and Propidium Iodide/RNase A solution for counter staining the total DNA. View All» Key Applications: Flow Cytometry View All» Species Reactivity: All View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Components:

• The APO-BRDU™ Kit is shipped in one container and consists of two packages. One package is shipped at ambient temperature and should be stored at 2-8°C upon arrival. The other package is styrofoam containing frozen ice packs and the reagent contents should be stored at -20°C upon arrival. Note: Upon arrival store the reagents at the appropriate temperatures.

• Reagent bottles have color coded caps to aid in their identification. Sufficient reagents are provided to process 60 cell suspensions including 5 mL positive and 5 mL negative control cell suspensions of approximately 1 x 106 cells per mL in 70% (v/v) ethanol. The control cells are derived from a human lymphoma cell line and have been fixed as described on page 6.

• Positive Control Cells, Brown cap

• Negative Control Cells, White cap

• Wash Buffer, Blue cap

• Reaction Buffer, Green cap

• TdT Enzyme, Yellow cap

• Br-dUTP, Violet cap

• Rinsing Buffer, Red cap

• Fluorescein~PRB-1 mAb, Orange cap

• PI/RNase Staining Buffer, Amber bottle
  View All» Brand Family: Chemicon View All» Storage Conditions: Precautions and Warnings 1. The components of this kit are for Research Use only and are not intended for diagnostic procedures. 2. Positive and negative control cells contain 70% (v/v) ethanol as a preservative; wash and reaction buffer contain sodium cacodylate (dimethylarsinic) as a buffer; rinsing and PI/RNase staining buffer contain 0.05% (w/v) sodium azide as a preservative. These materials are harmful if swallowed; avoid skin contact, wash immediately with water. See Material Safety Data Sheets. 3. TdT Enzyme will not freeze at -20oC, because it is in 50% (v/v) glycerol solution. Upon warming the TdT enzyme solution, centrifuge the tube for 30 seconds to force all the liquid to the bottom of the tube. View All» Promotional Text: Special Offer on Antibodies! Click Here! View All» Detection Methods: Fluorescent View All» Product Name: Apo-BrdU™ Flow Cytometry Detection Kit View All» Materials Required but Not Delivered: 1. Flow Cytometer 2. Distilled Water 3. 1% (w/v) paraformaldehyde (methanol free) in PBS 4. 70% (v/v) ethanol 5. 37°C Water Bath 6. Ice Bucket 7. 12 x 75 mm flow cytometry test tubes 8. Micro-pipettor View All»
  © 金山科研平台是专业的授权总代理区域代理经销平台。
  © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
  © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
  © 本文地址：https://www.16ao.com/thread-160976.htm
  © Merck Millipore代理APT115 APO-BRDU-50T，产品报价联系微信jinshanbio