货号：APT165

品牌：Merck Millipore

规格：100assays

目录价：￥8361.00

市场价格：￥7106.85

会员价格：￥6688.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Caspase 3 Colorimetric Activity Assay Kit, DEVD View All» Trade Name: Chemicon (Millipore) View All» Qty/Pk: 100 assays View All» Product Overview: Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's Caspase-3 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The free pNA can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3 activity. View All» Key Applications: Activity Assay View All» Species Reactivity: Mammals View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Entrez Gene Summary: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. View All» UniProt Summary: FUNCTION: SwissProt: P42574 # Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.SIZE: 277 amino acids; 31608 Da SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit.SUBCELLULAR LOCATION: Cytoplasm.TISSUE SPECIFICITY: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.PTM: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.SIMILARITY: SwissProt: P42574 ## Belongs to the peptidase C14 family. View All» Components:

• 5X Cell Lysis Buffer (Part No. 90065): 5 mL

• 5X Assay Buffer (Part No. 90066): 10 mL

• Caspase-3 Substrate (Ac-DEVD-pNA) (Part No. 90080): 1 mL of 3 mg/mL solution

• Caspase-3 Inhibitor (Ac-DEVD-CHO) (Part No. 90067): 50 μL of 100 μM (0.05 mg/mL) in DMSO

• pNA Standard (Part No. 90085): 250 μL of 10 mM in DMSO
  View All» Brand Family: Chemicon View All» UniProt Number: P42574 View All» Storage Conditions: Store kit materials at -20°C up to their expiration date. Special Precautions: · After thawing reagents, use immediately or aliquot and freeze at -20oC for longer storage. Avoid repeated freeze/thaw cycles. · The Caspase-3 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers. View All» Detection Methods: Chromogenic View All» Gene Symbol:
  • CASP3

  • apopain

  • procaspase3

  • CPP-32

  • Apopain

  • Yama

  • CASP-3

  • CPP32B

  • SCA-1

  • CPP32

  • EC 3.4.22.56 [Contains: Caspase-3 subunit p17

  • Caspase-3 subunit p12].
    View All» Product Name: Caspase 3 Colorimetric Activity Assay Kit, DEVD View All» Entrez Gene Number:
    • NM_004346.3

    • NM_032991.2
      View All» Alternate Names: CPP32 View All» Materials Required but Not Delivered: · Microcentrifuge and 1.5 mL Microcentrifuge tubes · 37°C Waterbath or Incubator · Spectrophotometer or Microplate Reader · 96 well Microtiter Plate View All»
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