产品英文名称: EasySep™ RosetteSep™ Complete Kit for CD4+CD25+ T Cells

产品中文名称: EasySep™ RosetteSep™ CD4+CD25+ T细胞完整试剂盒

EasySep™ RosetteSep™ Complete Kit for CD4+CD25+ T Cells 产品英文描述

The Complete Kit for Human CD4+CD25+ Regulatory T Cells is a simple two-step method that is designed to isolate CD4+CD25+ cells from whole blood or buffy coat. CD4+ T cells are enriched from whole blood or buffy coat by negative selection using RosetteSep® CD4+ T Cell Enrichment Cocktail. The RosetteSep® cocktail contains a combination of antibodies that is directed against non-CD4+ T cells and glycophorin A red blood cells (RBCs). When centrifuged over a buoyant density medium such as RosetteSep® DM-L (Catalog #15705) or Ficoll-Paque™ PLUS (Catalog #07957), the unwanted cells pellet along with the RBCs. The purified cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium. The enriched CD4+ T cells are then selected for CD25+ cells by positive selection using EasySep® Human CD25 Positive Selection Kit. Cells are targeted with Tetrameric Antibody Complexes recognizing CD25 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep® magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.

EasySep™ RosetteSep™ Complete Kit for CD4+CD25+ T Cells 推荐使用

The isolation of human CD4+CD25+ T cells from whole blood or buffy coat by positive selection

EasySep™ RosetteSep™ Complete Kit for CD4+CD25+ T Cells 产品组成

• RosetteSep™ Human CD4+ T Cell Enrichment Cocktail, 5 x 2 mL

• EasySep™ Human CD25 Positive Selection Cocktail, 1 mL

• EasySep™ Magnetic Particles, 1 mL

EasySep™ RosetteSep™ Complete Kit for CD4+CD25+ T Cells 保存方法

EASYSEP® HUMAN CD25 POSITIVE SELECTION COCKTAIL AND

EASYSEP® MAGNETIC NANOPARTICLES

Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 – 25°C), and should be refrigerated upon receipt.

EasySep™ RosetteSep™ Complete Kit for CD4+CD25+ T Cells 订购信息

Stemcell 15862 Complete Kit for Human CD4+CD25+ T Cells Immunomagnetic Positive Selection Kit Stemcell 15022 RosetteSep™ Human CD4+ T Cell Enrichment Cocktail Immunodensity Negative Selection Cocktail

Stemcell 18231 EasySep™ Human CD25 Positive Selection Kit Immunomagnetic Positive Selection Kit Stemcell 10512 CD25/IL-2R-alpha Antibody, Clone 2A3, PE-Conjugated Mouse Monoclonal Antibody to Human CD25 (IL-R2α) - PE Conjugated

Stemcell 07801 Lymphoprep™ Density Gradient Medium for the Isolation of Mononuclear Cells

Stemcell 15705 RosetteSep™ DM-L Density Medium Density Gradient Medium

Stemcell 18000 EasySep™ Magnet Immunomagnetic Column-Free Magnet

Stemcell 18001 The Big Easy EasySep™ Magnet Immunomagnetic Column-Free Magnet

Stemcell 20000 RoboSep™ RoboSep™ - The Fully Automated Cell Separator

For research use only. Not intended for human or animal diagnostic or therapeutic uses.