货号:AQ301F
品牌:Merck Millipore
规格:
目录价:¥2504.00
市场价格:¥2128.40
会员价格:¥2003.20
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Description:
Sheep anti-Rabbit IgG Antibody, F(ab')2, FITC conjugate | AQ301F
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Promotional Text:
Special Shipping Offer on Antibodies100% Performance Guaranteed
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Trade Name:
Chemicon (Millipore)
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Product Overview:
The reagent is an anti-rabbit antibody F(ab')2 fragment from sheep. The F(ab')2 fragments are obtained by pepsin cleavage. The fragments are coupled 5(6)-carboxyfluorescein-N-hydroxysuccinimide ester (fluorescein) and stabilized in buffer.The molar ratio of fluorescein/ protein is between 3 - 4. PURIFICATION: The antibody is isolated from antisera by immunoaffinity chromatography using antigen coupled to agarose beads.
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Specificity:
The reaction profile of the antibody was examined in an ELISA system: micro titer plates were coated with antigen and potentially cross-reacting IgG of other species in identical concentrations, incubated with anti-rabbit IgG-biotin and detected with a streptavidin-enzyme conjugate. In this system the antibody shows the following reaction profile: No cross-reactivity (FLUORESCEIN/PROTEIN MOLAR RATIO: Between 3 and 4.
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Species Reactivity:
Rabbit
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Application Notes:
The reagent allows a reliable and sensitive detection of rabbit IgG in immunohistochemistry and immunocytochemistry techniques. In addition, the absence of the "sticky" Fc portion avoids unspecific binding. Thus, the reagent is particularly suitable for use on sections and cells. RECOMMENDED CONCENTRATION: 20-50 μg/mL The concentrated stock solution (200 μg/mL) should be diluted with PBS, pH 7.1 containing 15 mg/mL BSA. Optimal working concentrations must be determined by the end user.
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Presentation:
Lyophilized. Buffer = 0.02 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.1% sodium azide. RECONSTITUTION: Reconstitute with 1 mL of sterile distilled water.
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Storage Conditions:
When stored protected from light, the lyophilized antibody-conjugate is stable at 2-8°C.When stored protected from light the reconstituted antibody conjugate solution is stable for 2 months at 2-8°CThe reconstituted solution should be stored in aliquots at -20°C; repeated freezing and thawing should be avoided.
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunofluorescence
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Brand Family:
Chemicon
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Product Name:
Sheep anti-Rabbit IgG Antibody, F(ab')2, FITC conjugate | AQ301F
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Antibody Type:
Polyclonal Antibody
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Qty/Pk:
200 µg
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Format:
FITC
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Host:
Sheep
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