货号：AQ301R

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Sheep anti-Rabbit IgG Antibody, F(ab')2, Rhodamine conjugate | AQ301R View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Product Overview: The reagent is an anti-rabbit antibody F(ab')2 fragment from sheep. The F(ab')2 fragments are obtained by pepsin cleavage. The fragments are coupled to 5(6)-carboxy-rhodamine 101-N-hydroxysuccinimide ester (rhodamine) and stabilized in buffer.The molar ratio of rhodamine: protein is between 2-3. PURIFICATION: The antibody is isolated from antisera by immunoaffinity chromatography using antigen coupled to agarose beads. View All» Specificity: The reaction profile of the antibody was examined in an ELISA system: micro titer plates were coated with antigen and potentially cross-reacting IgG of other species in identical concentrations, incubated with anti-rabbit IgG-biotin and detected with a streptavidin-enzyme conjugate. In this system the antibody shows the following reaction profile: No cross-reactivity (WAVE LENGTH: Absorption peak = 550 nm, Emission peak, 570 nm. View All» Species Reactivity: Rabbit View All» Application Notes: The reagent allows a reliable and sensitive detection of rabbit IgG immunohistochemistry, respectively immunocytochemistry, especially when interference of immunoglobulins of other species than rabbit may occur. In addition, the absence of the "sticky" Fc portion avoids unspecific binding. Thus, the reagent is particularly suitable for use on sections and cells. RECOMMENDED CONCENTRATION: 20-50 μg/mL. It is recommended that the antibody be diluted with PBS, pH 7.1 containing 15 mg/mL BSA. Optimal working concentrations must be determined by the end user. View All» Presentation: Lyophilized from 0.02M Sodium phosphate, 0.25M NaCl, pH 7.1 with 15 mg/mL BSA and 0.1% sodium azide. RECONSTITUTION: Reconstitute with 1 mL of sterile distilled water. View All» Storage Conditions: When stored protected from light, the lyophilized antibody-conjugate is stable at 2-8°C.When stored protected from light the reconstituted antibody conjugate solution is stable for 2 months at 2-8°CThe solution should be stored in aliquots at -20°C.Repeated freezing and thawing should be avoided. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Immunofluorescence View All» Brand Family: Chemicon View All» Product Name: Sheep anti-Rabbit IgG Antibody, F(ab')2, Rhodamine conjugate | AQ301R View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 200 µg View All» Format: Rhodamine (TRITC) View All» Host: Sheep View All»