金山科研平台，产品价格货期咨询微信：jinshanbio Assay Type:Solid Phase Sandwich ELISA Format:96-well strip plate Assay Length:4.5 hours Sample Type & Volume Required Per Well:Cell Culture Supernates (17 µL), Serum (17 µL), EDTA Plasma (17 µL) Sensitivity:3.5 pg/mL Assay Range:37.5 - 2,400 pg/mL (Serum, Cell Culture Supernates, EDTA Plasma) Specificity:Natural and recombinant mouse TIMP-1. This kit detects approximately 80% of the recombinant mouse TIMP-1 complexed with the pro-form of recombinant mouse MMP-9 and approximately 60% when complexed with active recombinant mouse MMP-9. Cross-reactivity: Interference:Interference observed with 1 or more available related molecules. Molecule Information: TIMP-1 Long Name: Tissue Inhibitors of Metalloproteinases 1 Entrez Gene IDs: 7076 (Human); 21857 (Mouse); 116510 (Rat) Background: TIMPs TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.