产品英文名称：Recombinant Human IFN‑-γ GMP

产品中文名称：重组人干扰素-γGMP

R&D 重组人干扰素-γGMP Recombinant Human IFN‑-γ GMP英文描述：

Predicted Molecular Mass: 16.9 kDa

SDS-PAGE: 17 kDa, reducing conditions

Activity: Measured in antiviral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach.

Clemens, M.J. et al. (eds): IRL Press. 129.

The ED50 for this effect is typically 0.15-0.75 ng/mL.

Formulation: Lyophilized from a 0.2 µm filtered solution in Sodium Succinate, Mannitol and Tween® 80. See Certificate of Analysis for details.

Source: E. coli-derived, Gln24-Gln166, with an N-terminal Met

Accession #: P01579

N-terminal Sequence Analysis: Met-Gln24-Asp-Pro-Tyr-Val-Lys-Glu-Ala-Glu

Purity: >97%, by SDS-PAGE with by silver staining, under reducing conditions.

Endotoxin Level: < 0.01 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass: 16.9 kDa

SDS-PAGE: 17 kDa, reducing conditions

Activity: Measured in antiviral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach.

Clemens, M.J. et al. (eds): IRL Press. 129.

The ED50 for this effect is typically 0.15-0.75 ng/mL.

Formulation: Lyophilized from a 0.2 µm filtered solution in Sodium Succinate, Mannitol and Tween® 80. See Certificate of Analysis for details.

Produced using non-animal reagents in an animal-free laboratory.

Manufactured and tested under current Good Manufacturing Practice (GMP) guidelines.

Reconstitution: Reconstitute at 200 µg/mL in sterile, deionized water.

Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

·12 months, -20 to -70 °C as supplied.

·1 month, 2 to 8 °C under sterile conditions after reconstitution.

·3 months, -20 to -70 °C under sterile conditions after reconstitution.

R&D 重组人干扰素-γGMP Recombinant Human IFN‑-γ GMP实验数据图像：

1 μg/lane of GMP-grade Recombinant Human IFN-gamma (Catalog#285-GMP) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.

GMP-grade Recombinant Human IFN-gamma (Catalog#285-GMP) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ is typically 0.15-0.75 ng/mL.

MALDI-TOF analysis of GMP-grade Recombinant Human IFN-gamma (Catalog#285-GMP). The major peak corresponds to the calculated molecular mass, 16881 Da. The minor peak at 17099 Da is a matrix-associated artifact of the MALDI-TOF.

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