ScienCellGK116人B细胞和T细胞发育标记qPCR检测试剂盒

2023-06-29

GK116 ScienCell 人B细胞和T细胞发育标记qPCR检测试剂盒,Human B and T Cells Development Markers qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞科研金山平台现货促销授权一级代理,咨询微信:jinshanbio 金山禾斗石开平台总代理的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNA,cDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

北京诺博莱德作为ScienCell品牌的特约代理商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK116 ScienCell人B细胞和T细胞发育标记qPCR检测试剂盒,Human B and T Cells Development Markers qPCR Array Kit,产品简介:

产品品牌: ScienCell

产品货号:GK116

产品名称:GK116 ScienCell人B细胞和T细胞发育标记qPCR检测试剂盒,Human B and T Cells Development Markers qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

GK116 ScienCell人B细胞和T细胞发育标记qPCR检测试剂盒,Human B and T Cells Development Markers qPCR Array Kit, 产品说明:

ScienCell 的 GeneQuery™ 人类 B 和 T 细胞发育标记 qPCR 阵列试剂盒 (GQH-BTD) 旨在促进参与常见淋巴祖细胞 (CLP) 分化为 B 细胞和 T 细胞的 40 个标记基因的基因表达谱。如何根据其功能对所包含的基因进行分组的简要示例如下所示:

CLP 标记物: MME, CD34, IL7R, FLT3

ProB-PreB-未成熟B细胞标志物:MME,MS4A1,SPN,PTPRC,IL7R,CD79A / B,CD40,CD19

B1 细胞标志物:CD19、MS4A1、CD27、SPN、CD40LG

调节性 B 细胞标志物:CD1D、CD5、CD19、CR2、CD24

边缘区B细胞标志物:CD1C,CD19,MS4A1,CR2,CD24

滤泡 B 细胞标志物:CD19、MS4A1、CR2、CD22、FCER2

记忆 B 细胞标志物:CD27、CD80、CD84、CD86

浆母细胞-浆细胞标志物:CD19、CD27、CD38、IL6R、SDC1、SLAMF7

双阴性和双阳性阶段 T 细胞标志物:CD1A、CD3D/E/G、CD4、CD5、CD7、CD8A/B、CD38、SELE/L/P、CD160、CD200

注意:所有基因名称都遵循人类基因组组织基因命名委员会(HGNC)的符号。

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可以特异性识别并有效扩增靶基因的 cDNA。精心设计的引物确保:(i)qPCR分析中的最佳退火温度为65°C(含2 mM Mg2+,不含DMSO);(ii)除非另有说明,否则引物组可识别靶基因的所有已知转录本变体;(iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human B and T Cells Development Markers qPCR Array Kit (GQH-BTD) is designed to facilitate gene expression profiling of 40 marker genes involved in the differentiation of common lymphoid progenitors (CLPs) into B and T cells. Brief examples of how included genes may be grouped according to their functions are shown below:

· CLP markers:MME, CD34, IL7R, FLT3

· ProB-PreB-immature B cell markers:MME, MS4A1, SPN, PTPRC, IL7R, CD79A/B, CD40, CD19

· B1 cell markers:CD19, MS4A1, CD27, SPN, CD40LG

· Regulatory B cell markers:CD1D, CD5, CD19, CR2, CD24

· Marginal Zone B cell markers:CD1C, CD19, MS4A1, CR2, CD24

· Follicular B cell markers:CD19, MS4A1, CR2, CD22, FCER2

· Memory B cell markers:CD27, CD80, CD84, CD86

· Plasmablast-plasma cell markers:CD19, CD27, CD38, IL6R, SDC1, SLAMF7

· Double-negative and double-positive stage T cell markers:CD1A, CD3D/E/G, CD4, CD5, CD7, CD8A/B, CD38, SELE/L/P, CD160, CD200

Note:all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查询™人类 B 细胞和 T 细胞发育标记 qPCR 阵列板布局*(8 个粗体和斜体控件)

注意:所有基因名称都遵循HGNC的符号

1

2

3

4

5

6

7

8

9

10

11

12

A

CD160

CD27

CD40LG

CD8A

MS4A1

ACTB

CD160

CD27

CD40LG

CD8A

MS4A1

ACTB

B

CD19

CD34

CD5

CD8B

PTPRC

GAPDH

CD19

CD34

CD5

CD8B

PTPRC

GAPDH

C

CD1A

CD38

CD7

CR2

SDC1

LDHA

CD1A

CD38

CD7

CR2

SDC1

LDHA

D

CD1C

CD3D

CD79A

FCER2

SELE

NONO

CD1C

CD3D

CD79A

FCER2

SELE

NONO

E

CD1D

CD3E

CD79B

FLT3

SELL

PPIH

CD1D

CD3E

CD79B

FLT3

SELL

PPIH

F

CD200

CD3G

CD80

IL6R

SELP

GDC

CD200

CD3G

CD80

IL6R

SELP

GDC

G

CD22

CD4

CD84

IL7R

SLAMF7

PPC

CD22

CD4

CD84

IL7R

SLAMF7

PPC

H

CD24

CD40

CD86

MME

SPN

NTC

CD24

CD40

CD86

MME

SPN

NTC

提示:基因选择可能会根据新的研究和开发进行更新

ScienCell基因表达分析产品订购信息:

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GK073 人类小胶质细胞Sensome qPCR检测试剂盒,Human Microglial Sensome qPCR Array Kit

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GK078 人类神经细胞生物学qPCR检测试剂盒,Human Neuronal Cell Biology qPCR Array Kit

GK079 人类软骨细胞生物学qPCR检测试剂盒,Human Chondrocyte Biology qPCR Array Kit

GK080 人成骨分化qPCR检测试剂盒,GeneQuery™ Human Osteogenic Differentiation qPCR Array Kit

GK081 人类周细胞生物学qPCR检测试剂盒,Human Pericyte Biology qPCR Array Kit

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GK093 Human Renal Mesangial Cell Biology qPCR Array Kit,Human Renal Mesangial Cell Biology qPCR Array Kit

GK094 Human Mesenchymal Stem Cell Differentiation qPCR Array,Human Mesenchymal Stem Cell Differentiation qPCR Array Kit

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