货号：550058

品牌：BD Pharmingen

规格：100ug

目录价：￥3338.00

市场价格：￥2102.94

会员价格：￥1835.90

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with 150 mM NaCl and 0.09% (w/v) sodium azide.

Preparation and Storage

The antibody was purified from tissue culture supernatant by affinity chromatography. The antibody solution should be stored undiluted at 4°C.

Usage

This antibody has been tested by immunofluorescent staining ( 1 mg/million cells) with flow cytometric analysis to assure specificity and reactivity. Other reported applications include ELISA of recombinant soluble Crry/p65 protein, in vitro blocking of Crry/p65 function,¹ and immunohistochemical staining (IHC) of zinc-fixed paraffin-embedded sections and acetone-fixed frozen sections.⁸ We do not recommend this antibody for western blot analysis or IHC of formalin-fixed paraffin-embedded sections. Since applications vary, each investigator must determine dilutions appropriate for individual use.

Caution

Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format for in vitro and in vivo use.

Conditions

BD PharMingen will not be responsible for violations or patent infringements which may occur with the use of our products.

Catalog Number

550058

Brand

BD Pharmingen™

Name

Crry/p65

Concentration

0.5 mg/ml

Size

0.1mg

RegulatoryStatus

RUO

Clone

1F2

Format

Purified

Isotype

IgG_2a, κ

Immunogen

Recombinant soluble Crry/p65 protein

Strain Host

SD

Reactivity

QC Testing : Mouse

Applications

ELISA Capture	- Reported
Flow cytometry	- Routinely Tested
Functional assay	- Reported
Immunohistochemistry-frozen	- Reported
Immunohistochemistry-paraffin	- Not Recommended
Immunohistochemistry-zinc-fixed	- Reported

Clone - References

1. Li, B., C. Sallee, M. Dehoff, S. Foley, H. Molina, and V.M. Holers. 1993. Mouse Crry/p65. Characterization of monoclonal antibodies and the tissue distribution of a functional homologue of human MCP and DAF. J. Immunol. 151: 4295 - 4305.2. Molina, H., W. Wong, T. Kinoshita, C. Brenner, S. Foley, and V.M. Holers. 1992. Distinct receptor and regulatory properties of recombinant mouse complement receptor 1 (CR1) and Crry, the two genetic homologues of human CR1. J. Exp. Med. 175: 121 - 129.3. Foley, S., B. Li, M. Dehoff, H. Molina, and V.M. Holers. 1993. Mouse Crry/p65 is a regulator of the alternative pathway of complement activation. Eur. J. Immunol. 23: 1381 - 1384.4. Kurtz, C.B., E. O'Toole, S.M. Christensen, and J.H. Weis. 1990. The murine complement receptor gene family. IV. Alternative splicing of Cr2 gene transcripts predicts two distinct gene products that share homologous domains with both human CR2 and CR1. J. Immunol. 144: 3581 - 3591.5. Holers, V.M., T. Kinoshita, and H. Molina. 1992. The evolution of mouse and human complement C3-binding proteins: divergence of form but conservation of function. Immunol. Today 13: 231 - 236.6. Martin, B.K., and J.H. Weis. 1993. Murine macrophages lack expression of the Cr2-145 (CR2) and Cr2-190 (CR1) gene products. Eur. J. Immunol. 23: 3037 - 3042.7. Xu, C., D. Mao, V.M. Holers, B. Palanca, A.M. Cheng, and H. Molina. 2000. A critical role for murine complement regulator Crry in fetomaternal tolerance. Science 287: 498 - 501.8. BD PharMingen. Unpublished results.

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