货号：550275

品牌：BD Pharmingen

规格：1EA

目录价：￥3793.00

市场价格：￥2389.59

会员价格：￥2086.15

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Purified immunoglobulin in IHC antibody diluent containing serum* and 0.09% (w/v) sodium azide.

Preparation and Storage

Preparation: The antibody was purified from ascites or tissue culture supernatant by affinity chromatography. Storage: The antibody solution should be stored undiluted at 4°C.

Caution

Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Conditions

The information disclosed herein is not to be construed as a recommendation to use the above product in violation of any patents. PharMingen will not be held responsible for patent infringement or other violations that may occur with the use of our products.

Catalog Number

550275

Brand

BD Pharmingen™

Name

CD3e

Alias

Cd3e, AI504783, CD3, CD3epsilon, T3e CD3 ε chain

Concentration

31.25 µg/ml

Size

1mL

RegulatoryStatus

RUO

Clone

145-2C11

Format

Purified

Isotype

IgG₁, κ

W.B. Positive Control

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Immunogen

H-2Kb specific cytotoxic T lymphocyte clone BM10-37

Reactivity

QC Testing : Mouse

Applications

(Co)-stimulation	- Tested During Development
Blocking	- Reported
Flow cytometry	- Routinely Tested
Immunohistochemistry-formalin (antigen retrieval required)	- Not Recommended
Immunohistochemistry-frozen	- Tested During Development
Immunohistochemistry-zinc-fixed	- Tested During Development
Immunoprecipitation	- Reported
Western Blot	- Reported

Clone - References

1. Leo, O., M. Foo, D.H. Sachs, L.E. Samelson, and J.A. Bluestone. 1987. Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proc. Natl. Acad. Sci. USA 84: 1374 - 1378.2. Isakov, N., R.L. Wange, W.H. Burgess, J.D. Watts, R. Aebersold, and L.E. Samelson. 1995. ZAP-70 binding specificity to T cell receptor tyrosine-based activation motifs: The tandem SH2 domains of ZAP-70 bind distinct tyrosine-based activation motifs with varying affinity. J. Exp. Med. 181: 375 - 380.3. Nakano, H., T. Yamazaki, S. Miyatake, N. Nozaki, A. Kikuchi, and T. Saito. 1996. Specific interaction of topoisomerase II™ and the CD3e chain of the T cell receptor complex. J. Biol. Chem. 271: 6483 - 6489.4. Portoles, P., J. Rojo, A. Golby, M. Bonneville, S. Gromkowski, L. Greenbaum, C.A. Janeway, Jr., D.B. Murphy, and K. Bottomly. 1989. Monoclonal antibodies to murine CD3- define distinct epitopes, one of which may interact with CD4 during T cell activation. J. Immunol. 142: 4169 - 4175.5. Shinkai, Y., and F.W. Alt. 1994. CD3-mediated signals rescue the development of CD4+CD8+ thymocytes in RAG-2-/- mice in the absence of TCR ™ chain expression. Int. Immunol. 6: 995 - 1001.6. Radvanyi, L.G., G.B. Mills, and R.G. Miller. 1993. Religation of the T cell receptor after primary activation of mature T cells inhibits proliferation and induces apoptotic cell death. J. Immunol. 150: 5704 - 5715.7. Castro, J.E., J.A. Listman, B.A. Jacobson, Y. Wang, P.A. Lopez, S. Ju, P.W. Finn, and D.L. Perkins. 1996. Fas modulation of apoptosis during negative selection of thymocytes. Immunity 5: 617 - 627.8. Salvadori, S., B. Gansbacher, A.M. Pizzimenti, and K.S. Zier. 1994. Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors. J. Immunol. 153: 5176 - 5182.

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