货号：550350

品牌：BD Pharmingen

规格：1EA

目录价：￥3200.00

市场价格：￥2016.00

会员价格：￥1760.00

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with 150 mM NaCl and 0.09% (w/v) sodium azide.

Preparation and Storage

The antibody was purified from tissue culture supernatant by affinity chromatography. The antibody solution should be stored undiluted at 4°C.

Usage

This antibody has been tested by immunofluorescent staining ( 1 mg/million cells) with flow cytometric analysis to assure specificity and reactivity. We recommend the use of Mouse Fc Block™ (anti-mouse CD16/CD32, mAb 2.4G2, Cat. no. 553141/553142) to reduce non-specific staining. For staining in the presence of Mouse Fc Block™, the second-step antibody must not recognize the 2.4G2 antibody (rat IgG_2b, k). Therefore, we recommend biotinylated mouse anti-rat IgG_2a (Cat. no. 553894) as a secondary antibody, followed by Streptavidin-PE (Cat. no. 554061). Since applications vary, each investigator must determine dilutions appropriate for individual use.

Caution

Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format for in vitro and in vivo use.

Conditions

BD PharMingen will not be responsible for violations or patent infringements which may occur with the use of our products.

Catalog Number

550350

Brand

BD Pharmingen™

Name

Syndecan-4

Concentration

0.5 mg/ml

Size

0.1mg

RegulatoryStatus

RUO

Clone

KY/8.2

Format

Purified

Isotype

IgG_2a, κ

W.B. Positive Control

none

Immunogen

BCB10 mouse pre-B cell line

Strain Host

WI

Reactivity

QC Testing : Mouse

Applications

ELISA	- Reported
Flow cytometry	- Routinely Tested

Clone - References

1. Yamashita, Y., K. Oritani, E.K. Miyoshi, R. Wall, M. Bernfield, and P.W. Kincade. 1999. Syndecan-4 is expressed by B lineage lymphocytes and can transmit a signal for formation of dendritic processes. J. Immunol. 162: 5940 - 5948.2. Rapraeger, A.C., and V.L. Ott. 1998. Molecule interactions of the syndecan core proteins. Curr. Opin. Cell Biol. 10: 620 - 628.