货号：550380

品牌：BD Pharmingen

规格：1mg

目录价：￥6988.00

市场价格：￥4402.44

会员价格：￥3843.40

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lyophilized

Preparation and Storage

Reconstitute 1.0 mg of Z-IETD- FMK in DMSO. We typically make a 10 mM stock solution (dissolve 1.0 mg of Z-IETD-FMK in 150 µl DMSO). The final concentration of inhibitor may vary between experimental systems and therefore should be optimized by the user. As a precautionary note, please do not exceed a final DMSO concentration of 0.2% because levels above this may cause some cellular toxicity and mask the effectof the caspase inhibitor. Store the lyophilized inhibitor at -20°C. Reconstitute the inhibitor in DMSO before use. Store the reconstituted inhibitor in small aliquots at -20°C and avoid repeated freeze-thaw cycles.

References

1. Thornberry, N.A. andY. Lazebnik. 1998. Caspases: enemies within. Science 281:1312-1316.2. Gregoli, P.A. and M.C. Bondurant. 1999. Function of caspases in regulating apoptosis caused by erythropoietin deprivation in erythroid progenitors.J. Cell Physiol. 178:133-143.3. Wang, J., L. Zhen, M.G. Klug, D. Wood, X. Wu, and J.Mizrahi. 2000. Involvement of caspase 3- and 8-like proteases in ceramide-induced apoptosis ofcardiomyocytes. J. Card. Fail. 6:243-249.

Datasheet

Cat. No. 550377/69361U, Z-VAD-FMK, General Caspase InhibitorCat. No. 550378/69381U, Z-DEVD-FMK, Caspase-3 InhibitorCat. No. 550379/69391U, Z-VEID-FMK, Caspase-6 InhibitorCat. No. 550381/69411U, Z-LEHD-FMK, Caspase-9 InhibitorCat. No. 550411/69421U, Z-FA-FMK, Negative Caspase InhibitorFigure Legend: Flow cytometric analysis of Jurkat T cells. Jurkat cells were preincubated with the following: no inhibitor, 20 µM Z-IETD-FMK or 20 µM control inhibitor (Z-FA-FMK) for 30 min, and then either left untreated or treated with 4 µM of campthothecin for 3 hr. Following incubation, cells were collected and stained with Annexin V-PE (Cat. No. 559763/6900KK) to identify cells undergoing apoptosis. The results indicate that in campthothecin treated cells approximately 42% of the cellswere induced to undergo apoptosis and the use of the caspase-8 inhibitor Z-IETD -FMK reduced the level of apoptosis to that observed in untreated controls. Cells treated with Z-FA-FMK (Cat. No. 550411/69421U) showed similar results to the treated cells without inhibitor, indicating that the control inhibitor did not attenuate apoptosis.

Catalog Number

550380

Brand

BD Pharmingen™

Name

Caspase-8 Inhibitor, Z-IETD-FMK

Size

1mg

RegulatoryStatus

RUO

Format

Purified