货号：550519

品牌：BD Pharmingen

规格：100ug

目录价：￥2841.00

市场价格：￥1789.83

会员价格：￥1562.55

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Aqueous buffered solution containing 0.09% Sodium Azide.

Preparation and Storage

The antibody was purified from tissue culture supernatant by affinity chromatography. The antibody solution should be stored undiluted at 4C.

References

1. Vance, R.E., A.M. Jamieson, and D.H. Raulet. 1999. Recognition of the class Ib molecule Qa-1 b by putative activating receptorsCD94/NKG2C and CD94/NKG2E on mouse natural killer cells. J. Exp. Med. 190: 1801 1812.2. Vance, R.E. Personal communication.3. PharMingen. Unpublished results.4. Vance, R.E., J.R. Kraft, J.D. Altman, P.E. Jensen, and D.H. Raulet. 1998. Mouse CD94/NKG2A is a natural killer cell receptor forthe nonclassical major histocompatibility complex (MHC) class I molecule Qa-1 b . J. Exp. Med. 188: 1841 1848.5. Kubota, A., S. Kubota, S. Lohwasser, D.L. Mager, and F. Takei. 1999. Diversity of NK cell receptor repertoire in adult and neonatalmice. J. Immunol. 163: 212 216.6. Lohwasser, S., P. Hande, D.L. Mager, and F. Takei. 1999. Cloning of murine NKG2A, B and C: second family of C-type lectinreceptors on murine NK cells. Eur. J. Immunol. 29: 755 761.7. Yokoyama, W.M. 1998. Natural killer cell receptors. Curr. Opin. Immunol. 10: 298 305.Conditions: PharMingen will not be responsible for violations or patent infringements which may occur with the use of our products.

Caution

Caution: Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format for in vitro and in vivo use.

Datasheet

Expression of NKG2A/C/E on mouse splenic NK cells. C57BL/6 splenocytes weresimultaneously stained with PE-conjugated PK136 (anti-mouse NK-1.1, Cat. no.557391, both panels) and purified 20d5 (Panel B) monoclonal antibodies, followed byFITC-conjugated RG7/1.30 mAb (anti-rat IgG2a , Cat. no. 553896, both panels). TheNK1.1 + NKG2 + and NK1.1 + NKG2 - subsets are detected. Flow cytometry wasperformed on a FACSCalibur

Catalog Number

550519

Brand

BD Pharmingen™

Name

NKG2A/C/E

Concentration

0.5 mg/ml

Size

0.1mg

RegulatoryStatus

RUO

Clone

20d5

Format

Biotin

Isotype

IgG_2a, κ

W.B. Positive Control

none

Immunogen

Transfected cell line

Strain Host

LEW

Reactivity

QC Testing : Mouse

Applications

Flow cytometry

- Routinely Tested