货号：550738

品牌：BD Pharmingen

规格：1EA

目录价：￥1810.00

市场价格：￥1140.30

会员价格：￥995.50

金山科研平台，产品价格货期咨询微信：jinshanbio Contents

Purified immunoglobulin in 10 mM phosphate buffer, pH 7.2 with 150 mM NaCl and 0.09% (w/v) sodium azide.

Preparation and Storage

Preparation: The antibody was purified from tissue culture supernatant by affinity chromatography.Storage: The antibody solution should be stored undiluted at 4°C.

Usage

This antibody has been tested by immunofluorescent staining ( 1 mg/10⁶ cells) with flow cytometric analysis to assure specificity and reactivity. Other reported applications include inhibition of 5' NT enzyme activity,¹ immunohistochemical staining (IHC) of acetone-fixed frozen^1,3 and zinc-fixed paraffin-embedded³ sections, and costimulation of T lymphocytes, but not B lymphocytes.¹ IHC of formalin-fixed paraffin-embedded sections is not recommended.³ Since applications vary, each investigator must determine dilutions appropriate for individual use.

Caution

Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format for in vitro and in vivo use.

Conditions

BD PharMingen will not be responsible for violations or patent infringements which may occur with the use of our products.

Catalog Number

550738

Brand

BD Pharmingen™

Name

CD73

Alias

Ecto-5ʼ-nucleotidase

Concentration

0.5 mg/ml

Size

0.1mg

RegulatoryStatus

RUO

Clone

TY/23

Format

Purified

Isotype

IgG_2a, κ

W.B. Positive Control

none

Immunogen

BALB/c mouse splenocytes and CHO cells transfected with the Mouse CD73 gene, Nt5

Strain Host

WI

Reactivity

QC Testing : Mouse

Applications

Flow cytometry	- Routinely Tested
Immunohistochemistry-frozen	- Reported
Immunohistochemistry-paraffin	- Not Recommended
Immunohistochemistry-zinc-fixed	- Reported

Clone - References

1. Yamashita, Y., S.W. Hooker, H. Jiang, A.B. Laurent, R. Resta, K. Khare, A. Coe, P.W. Kincade, and L.F. Thompson. 1998. CD73 expression and fyn-dependent signaling on murine lymphocytes. Eur. J. Immunol. 28: 2981 - 2990.2. Resta, R., Y. Yamashita, and L.F. Thompson. 1998. Ecto-enzyme and signaling functions of lymphocyte CD73. Immunol. Rev. 161: 95 – 109.3. BD PharMingen. Unpublished results.

Images