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MesenCult™-ACF Chondrogenic Differentiation Medium is animal component-free (ACF) and specifically formulated for the in vitro differentiation of human mesenchymal stromal cells (MSCs; also known as mesencyhymal stem cells) into chondrogenic lineage cells, including chondrocytes. This medium is suitable for the differentiation of human bone marrow (BM)-, adipose tissue (AT)- and synovium (S)-derived MSCs previously culture-expanded in serum-containing medium (e.g. MesenCult™ Proliferation Kit [Catalog #05411]) or animal component-free MesenCult™-ACF Plus Medium [Catalog #05445]). MesenCult™-ACF Chondrogenic Differentiation Medium induces robust chondrogenic differentiation of human MSCs with as few as 3 x 10^5 cells and as early as day 14. Advantages Animal component-free (ACF) formulation Robust chondrogenic differentiation with as few as 3 x 10^5 MSCs and as early as day 14 Strong expression of chondrogenic transcripts - Acan, Col2a, Sox9 and Col10a; low expression of hypertrophic transcript Mmp13 Completes optimized ACF workflow for MSC isolation, expansion, cryopreservation and chondrogenic differentiation Components

• MesenCult™-ACF Chondrogenic Differentiation Basal Medium, 95 mL

• MesenCult™-ACF 20X Chondrogenic Differentiation Supplement, 5 mL

Subtype 专业培养基 Cell Type 软骨细胞, 间充质干细胞和祖细胞 Species 人 Application 分化, 细胞培养 Brand MesenCult Area of Interest 干细胞生物学 Formulation 无动物源成分, 无血清