TAKARA货号Y40001-TAKARA Y40001 RHB-A神经干细胞分化培养基

2025-08-03

Neural Stem Cell Culture Media
RHB-A和RHB-Basal是两款无血清、成分完全确定的培养基,用于人类或小鼠神经干细胞(NS)的定向分化获取、维持培养、扩增、诱导分化成神经细胞等用途。RHB-A可用来培养扩增贴壁的人或小鼠的神经干细胞。在RHB-A中培养的神经干细胞保持着稳定的神经干细胞分化发育成为成熟的神经细胞的能力 (1-3) 。另外,RHB-A还用来定向分化小鼠胚胎干细胞(mouse ES)至神经前体细胞 (neural precursors) (4-6)。 RHB‐Basal不包含生长因子或是神经细胞相关的添加物。因此,可以RHB‐Basal为基础,通过添加客户认为需要的添加物来开发适合客户的特殊类型细胞的培养基。
■ 产品特点
· RHB-A is a proprietary, fully defined, and serum-free medium designed to maintain pure populations of adherent human and mouse NS cells · RHB-Basal medium is animal component-free and contains no neuronal supplements; the media can be customized by the addition of supplements
■ 产品应用
· Derivation of mouse and human NS cells from ES cells and fetal and tissues · Maintenance and propagation of adherent mouse and human NS cells · Differentiation of mouse and human NS cells into functional neurons · Differentiation of mouse ES cells to neuronal precursors · Refer to the Data Sheet for additional examples of use
■ 产品详情请点击:
Adherent mouse neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express neural stem cell markers, including Nestin, Vimentin (3CB2), radial glial cell marker-2 (RC2), glial fibrillary acidic protein (GFAP), and microtubule associate protein (MAP).
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