乳果糖检测试剂盒
英文名:Lactulose Assay Kit
货号:MK-LACTUL
规格:50 assays
品牌: Megazyme
报价:¥3700.00
商品描述
乳果糖快速检测方法—Megazyme乳果糖快速检测试剂盒
Megazyme乳果糖检测试剂盒采用酶法分析原理,可定量检测鲜奶、UHT奶、浓缩乳和奶粉等各种乳品中的乳果糖。
与农业部新标准采用相同的检测原理和方法
该试剂盒采用ISO方法11285:2004,经过改进后,更加快速和灵敏
灵敏度是传统己糖激酶法的两倍
成本低
试剂配制后可稳定保存2年
采用试剂盒形式,包含检测必需的所有酶
提供计算软件,使数据处理更方便
包含标准品
The Lactulose Assay Kit is suitable for the specific, rapid and sensitive measurement and analysis of lactulose in milk and milk-based samples. Reagents included in this kit may also be prepared for use in the procedure described by ISO Method 11285:2004.
UV-method for the determination of Lactulose in milk and foodstuffs containing dairy products Principle: (β-galactosidase) (1) Lactulose + H2O → D-galactose + D-fructose (glucose oxidase + catalase + H2O2) (2) D-Glucose + H2O + O2 → D-gluconic acid + H2O2 (hexokinase) (3) D-Fructose + ATP → F-6-P + ADP (phosphoglucose isomerase) (4) F-6-P → G-6-P (glucose-6-phosphate dehydrogenase) (5) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+ (gluconate-6-phosphate dehydrogenase) (6) Gluconate-6-phosphate + NADP+ → ribulose-5-phosphate + NADPH + CO2 + H+
Kit size: 50 assaysMethod: Spectrophotometric at 340 nmReaction time: ~ 120 minDetection limit: 4.8 mg/LApplication examples:Milk, dairy products and foods containing milkMethod recognition: Novel method
Advantages
- Twice the sensitivity of traditional hexokinase based lactulose methods
- Very cost effective
- All reagents stable for > 2 years after preparation
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
文档
EK-LACTUL Data Booklet.pdf:
EK-LACTUL FAQ.pdf:
m.catalog.equl.cn.pdf:
视频
金山科研平台 Megazyme 乳果糖检测试剂盒操作视频 (K-LACTUL)
Megazyme 溶解淀粉 操作视频
Megazyme 试剂盒样品前处理准备操作视频
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