CD86, Human, Recombinant, 0.1 mg
英文名：CD86, Human, Recombinant, 0.1 mg
货号：BM-5096-0.1MG
规格：0.1 mg
品牌： Advanced Biomatrix
报价：￥2050.00
商品描述
Human CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms. Recombinant CD86 may service as coating matrix protein for studying of T cell functionsin vitro.
Full-length human CD86 extracellular domain cDNA (24 – 247 aa, Isoform-2) was constructed with 31 N-terminal T7/His tag and expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified as soluble protein
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Parameter, Testing, and Method
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CD86, Human, Recombinant Catalog # 5096
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Quantity
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0.1 mg (100 µg/vial)
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Volume
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0.2 mL
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Concentration
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0.5 mg/mL
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Purity
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>90% as measured by SDS PAGE
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Formulation
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Formulated in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, L-Arginine, DTT and Glycerol.
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Form
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Solution
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Production Type
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Recombinant – E. coli
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Storage Temperature
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Keep at -20°C for long term storage. Product is stable at 4 °C for at least 30 days
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Shelf Life
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12 months after receipt
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Sterilization Method
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Filtration
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Cell Attachment Activity
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Passes
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Sterility
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No growth
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Accession Number
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NP_008820
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Recombinant Protein Sequence
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MASMTGGQQMGRGHHHHHHGNLYFQGGEFELPLKIQAY FNETADLPCQFANSQNQSLSELVVFWQDQENLVLNEVYL GKEKFDSVHSKYMGRTSFDSDSWTLRLHNLQIKDKGLY QCIIHHKKPTGMIRIHQMNSELSVLANFSQPEIVPISNITE NVYINLTCSSIHGYPEPKKMSVLLRTKNSTIEYDGIMQKS QDNVTELYDVSISLSVSFPDVTSNMTIFCILETDKTRLLS SPFSIELEDPQPPPDHIP |
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