禽脑脊髓炎病毒抗体检测试剂盒

货号：AEV-2P

规格：184 test samples

品牌：金山科研平台

报价：￥2700.00

Kit contains approximately184 test samples plus controls, contains2 microtiter plates.

禽脑脊髓炎（AE），也被称为流行性震颤。声发射技术是通过鸡蛋传播主要是水平传播，虽然可以感染鸡和易感鸡或成熟的鸟类之间发生。年轻的小鸡（1～4周）表现出症状，如共济失调，共济失调，震颤麻痹，或快速的头部和颈部，而成年鸟有降低鸡蛋产量1。血清中的禽流感病毒抗体可通过酶联免疫吸附试验（ELISA）检测。血清中的抗原抗体水平，以及整个鸡群免疫状态可监测使用ELISA。

灭活抗原绑定到微孔。稀释的血清加入微孔培养。任何抗AEV抗体存在于血清中，将绑定到的微孔形成AEV抗原抗体复合物的抗原。洗去任何未结合的物质后，抗鸡IgG碱性磷酸酶缀合物加入到微孔。共轭将绑定到AEV抗原抗体复合物。孵育后洗涤除去任何未结合的。酶的底物，然后添加到微孔。基板有碱性磷酸酶存在反应并形成黄色产物。在最后一步反应停止，固定的颜色。颜色的强度是在405 – 410 nm光度测量。

Avian Encephalomyelitis (AE), is also known as Epidemic Tremor. AE is mainly transmitted through eggs, although horizontal transmission can occur between infected chicks and susceptible chicks or mature birds. Young chicks (1 to 4 weeks) exhibit symptoms such as ataxia, incoordination, paralysis, or rapid tremors of head and neck, while adult birds have decreased egg production1. Antibodies to avian viruses in serum can be detected by the Enzyme Linked Immunosorbant Assay (ELISA). The levels of AEV antibodies in serum, as well as the immune status of entire flocks can be monitored using ELISA.

Inactivated antigen is bound to a microwell. Diluted sera is added to the microwell and incubated. Any anti-AEV antibodies present in the serum will bind to the AEV on the microwell forming an AEV antigen-antibody complex. After washing away any unbound material, anti-Chicken IgG Alkaline Phosphatase conjugate is added to the microwell. The conjugate will bind to the AEV antigen-antibody complex. After incubation any unbound conjugate is removed by washing. Enzyme substrate is then added to the microwell. The substrate reacts with any Alkaline Phosphatase present and forms a yellow product. In the final step the reaction is stopped, fixing the color. The intensity of the color is measured photometrically at 405 – 410 nm.